• JoomlaWorks Simple Image Rotator
  • JoomlaWorks Simple Image Rotator
  • JoomlaWorks Simple Image Rotator
  • JoomlaWorks Simple Image Rotator
  • JoomlaWorks Simple Image Rotator
  • JoomlaWorks Simple Image Rotator
  • JoomlaWorks Simple Image Rotator
  • JoomlaWorks Simple Image Rotator
  • JoomlaWorks Simple Image Rotator
  • JoomlaWorks Simple Image Rotator
 
  Bookmark and Share
 
 
Doctoral Thesis
DOI
10.11606/T.42.2010.tde-19052010-131337
Document
Author
Full name
Denise Aparecida Berti
Institute/School/College
Knowledge Area
Date of Defense
Published
São Paulo, 2010
Supervisor
Committee
Ferro, Emer Suavinho (President)
Chagas, Jair Ribeiro
Colquhoun, Alison
Gozzo, Fábio Cesar
Schechtman, Deborah
Title in Portuguese
Peptídeos intracelulares na obesidade e resistência à insulina
Keywords in Portuguese
Espectrometria de massas
Insulina (resistência)
Obesidade
Peptídeos
Tecido adiposo
Abstract in Portuguese
A hipótese de que peptídeos gerados como produtos de proteólise intracelular poderiam modular cascatas de sinalização, foi originalmente proposto pelo nosso grupo. Um estudo realizado por Heimann et al. (2005) mostrou que camundongos geneticamente modificados e submetidos à dieta hiperlipídica, apresentavam uma diferença no conteúdo intracelular de peptídeos e uma melhora da resistência à insulina. Neste trabalho, investigamos o conteúdo peptídico intracelular do tecido adiposo de animais que desenvolveram obesidade e resistência à insulina, após serem submetidos à dieta cafeteria. Duas sequências peptídicas apresentaram 100% de aumento no tecido adiposo de animais submetidos à dieta cafeteria, em relação aos controles, tendo sido analisadas por ensaios de cinética enzimática, captação de glicose, western blot e cromatografia de afinidade. Os resultados obtidos corroboram os dados de Heimann et al. (2005) de que peptídeos intracelulares podem estar envolvidos na resistência à insulina, modulando o transporte de glicose no tecido adiposo.
Title in English
Intracellular peptides in obesity and insulin resistance
Keywords in English
Adipose tissue
Insulin (resistance)
Mass spectrometry
Obesity
Peptides
Abstract in English
The hypothesis that peptides generated as degradation products of intracellular proteolyses could modulate signaling pathways, was originally proposed by our group. A study by Heimann et al. (2005) showed that mice genetically modified and fed with high-fat diet, showed a difference in intracellular content of peptides and an improvement of insulin resistance. In this study, we investigated the intracellular peptide content from adipose tissue of animals that developed obesity and insulin resistance after being treated with the Western diet. Two peptide sequences showed 100% increase in adipose tissue of animals submitted to the Western diet compared to controls, and were analyzed by enzymatic assays, glucose uptake, western blot and affinity chromatography. Altogether, these results corroborate previous suggestions that intracellular peptides may be involved in insulin resistance, modulating glucose transport in adipose tissue.
 
WARNING - Viewing this document is conditioned on your acceptance of the following terms of use:
This document is only for private use for research and teaching activities. Reproduction for commercial use is forbidden. This rights cover the whole data about this document as well as its contents. Any uses or copies of this document in whole or in part must include the author's name.
Publishing Date
2010-06-08
 
WARNING: Learn what derived works are clicking here.
All rights of the thesis/dissertation are from the authors
Centro de Informática de São Carlos
Digital Library of Theses and Dissertations of USP. Copyright © 2001-2019. All rights reserved.