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Master's Dissertation
DOI
10.11606/D.42.2011.tde-22072011-155029
Document
Author
Full name
Loreni Cristine da Silva
Institute/School/College
Knowledge Area
Date of Defense
Published
São Paulo, 2011
Supervisor
Committee
Hamassaki, Dania Emi (President)
Joselevitch, Christina
Yamanouye, Norma
Title in Portuguese
A GtPase Rac1 participa da proliferação de células gliais de Müller após lesão excitotóxica.
Keywords in Portuguese
Ativação enzimática
Fosfolipases
Proliferação celular
Regeneração (fenômenos biológicos)
Retina
Transcrição gênica
Abstract in Portuguese
As células glias de Müller são capazes de gerar novos neurônios retinianos em resposta a lesões, atuando como uma possível fonte para regeneração retiniana. Nesse contexto, as GTPases Rho podem ter um papel interessante, visto que regulam múltiplas vias de sinalização que controlam, por exemplo, a transcrição gênica, sobrevivência e proliferação celular. No presente estudo analisamos a participação de um dos membros dessa família (Rac1) na proliferação de células gliais de Müller da retina de galinhas após lesão excitotóxica com N-Metil-D-Aspartato (NMDA). A injeção intraocular de NMDA promoveu extensa proliferação de células gliais de Müller. A inibição de Rac1 com NSC23766 não alterou a quantidade de células que entraram no ciclo celular, mas, provocou um retardo em sua progressão. Esses resultados sugerem um importante papel para a GTPase Rac1 na regulação da proliferação de células gliais de Müller em resposta a lesões retinianas.
Title in English
Rac1 GTPase participates in the proliferation of Müller glial cells after excitotoxic injury.
Keywords in English
Cell proliferation
Enzyme activation
Gene transcription
Phospholipase
Regeneration (the biological phenomena)
Retina
Abstract in English
Müller glial cells may generate new neurons in response to retinal injury, acting as a potential source for retinal regeneration. In this context, Rho GTPases may have an interesting role, since they regulate multiple signaling pathways that control, for example, gene transcription, cell proliferation and survival. This study analyzed the involvement of a member of this family (Rac1) in the proliferation of Müller glial cells of chick retina after excitotoxic injury with N-methyl-D-aspartate (NMDA). Intraocular injection of NMDA promoted extensive Müller glia proliferation. Rac1 inhibition with NSC23766 did not affect the cell cycle entry, but a delay in cell cycle progression was observed. These results suggest an important role for Rac1 in the regulation of Müller glial cells proliferation in response to retinal injury.
 
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Publishing Date
2011-08-24
 
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