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Master's Dissertation
DOI
10.11606/D.42.2010.tde-27092010-152352
Document
Author
Full name
Cesar Seigi Fuziwara
Institute/School/College
Knowledge Area
Date of Defense
Published
São Paulo, 2010
Supervisor
Committee
Kimura, Edna Teruko (President)
Chaves, Maria Luiza Morais Barreto de
Rubio, Ileana Gabriela Sanchez de
Title in Portuguese
Influência do MicroRNA let-7 e miR-17-92 como oncomiRs no câncer.
Keywords in Portuguese
Glândula tireóide
Let-7
MicroRNA
miR-17-92
Neoplasias da tireóide
Proliferação RET/PTC
Abstract in Portuguese
No câncer, alterações em microRNAs (miRNAs), pequenos RNAs que regulam a tradução protéica, exerce efeito oncogênico (oncomiR). Os oncomiRs regulam genes chave para a proliferação celular e apoptose, sendo importantes para a biologia do câncer. O carcinoma papilífero de tiróide apresenta alterações genéticas alinhadas na via MAPK (RET>RAS>BRAF>ERK). Observamos que a indução do oncogene RET/PTC diminui a expressão de let-7 em células foliculares tiroidianas. Na linhagem TPC-1 (com RET/PTC-1), a introdução de let-7 diminui a proliferação celular e a fosforilaçãode ERK, indicando papel de gene supressor tumoral. No carcinoma anaplásico, avaliamos o papel da introdução do cluster miR-17-92 na linhagem ARO. Observamos que in vitro miR-17-92 atua de forma oncogênica aumentando proliferação e viabilidade celular de ARO. No entanto, estas células apresentam diminuição no crescimento em soft-agar. No xenotransplante, os tumores de ARO-miR-17-92 apresentam menor volume e expressam MMP-9 de forma reduzida, indicando também um papel de gene supressor tumoral para o cluster.
Title in English
Influence of MicroRNA let-7 and miR-17-92 as oncomiRs in cancer.
Keywords in English
Let-7
MicroRNA
miR-17-92
Proliferation
RET-PTC
Thyroid gland
Thyroid neoplasms
Abstract in English
In cancer, alteration in microRNA, small RNAs (~22nt) that regulate post-transcriptionally protein levels, exerts oncogenic role (oncomiR). OncomiRs control genes involved in cell proliferation and apoptosis, influencing cancer biology. Papillary thyroid cancer displays activating genetic alterations in MAPK signaling pathway (RET>RAS>BRAF>ERK). Using conditional induction of oncogenes in thyroid cells, we observed that RET/PTC decreases let-7 miRNA expression. In papillary thyroid cancer cell TPC-1 (with RET/PTC-1) we observed that let-7 introduction inhibits cell proliferation and ERK phosphorylation, indicating tumor suppressor role for let-7. In anaplastic thyroid cancer, we evaluate the role of introduction of miR-17-92 cluster in ARO cell line. We observed in vitro that miR-17-92 increases ARO cell proliferation and viability, acting as oncogene. However, these cells show impaired soft agar growth. In xenotransplant, ARO-miR-17-92 tumors are smaller in volume and express reduced levels of MMP-9, indicating a tumor suppressor role for the cluster.
 
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Publishing Date
2010-10-20
 
WARNING: The material described below relates to works resulting from this thesis or dissertation. The contents of these works are the author's responsibility.
  • FUZIWARA, Cesar Seigi, et al. Effects of let-7 microRNA on cell growth and differentiation of papillary thyroid cancer. Translational Oncology, 2009, vol. 2, n. 4, p. 236-241.
All rights of the thesis/dissertation are from the authors
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