• JoomlaWorks Simple Image Rotator
  • JoomlaWorks Simple Image Rotator
  • JoomlaWorks Simple Image Rotator
  • JoomlaWorks Simple Image Rotator
  • JoomlaWorks Simple Image Rotator
  • JoomlaWorks Simple Image Rotator
  • JoomlaWorks Simple Image Rotator
  • JoomlaWorks Simple Image Rotator
  • JoomlaWorks Simple Image Rotator
  • JoomlaWorks Simple Image Rotator
 
  Bookmark and Share
 
 
Doctoral Thesis
DOI
10.11606/T.42.2016.tde-17112016-100306
Document
Author
Full name
Danilo de Oliveira Carvalho
Institute/School/College
Knowledge Area
Date of Defense
Published
São Paulo, 2016
Supervisor
Committee
Guimaraes, Margareth de Lara Capurro (President)
Andrioli, Luiz Paulo Moura
Fogaça, Andréa Cristina
Monesi, Nadia
Rocha, Lincoln Suesdek
Title in Portuguese
Obtenção e caracterização de linhagem transgênica de Aedes aegypti machos geneticamente estéreis.
Keywords in Portuguese
Aedes aegypti
Esterilidade condicional
Supressão populacional
Transgênico
Abstract in Portuguese
Com o aumento progressivo do número de casos de infecção por diferentes arbovírus, por exemplo, dengue, zica e chikungunya, faz-se necessário o desenvolvimento de novas técnicas para o combate a esses arbovírus. A manipulação genética possibilitou a obtenção de mosquitos geneticamente modificados que sejam capazes de suprimir a população selvagem ou impedir a transmissão de agentes etiológicos gerando doenças. O estudo teve como objetivo o estabelecimento de linhagens para supressão populacional de Aedes aegypti. Esta construção apresenta esterilidade condicionada à presença ou ausência de antibiótico no meio em que esses mosquitos se desenvolvem durante a fase larval. Dessa forma, sem a necessidade de se utilizar radiação para obter insetos estéreis, é possível melhorar a qualidade dos machos adultos liberados e aumentar a competitividade dos mesmos em competir por fêmeas selvagens e adicionalmente gerar o desejado quadro de supressão populacional.
Title in English
Obtention and characterization of transgenic lines of Aedes aegypti for males genetically sterile.
Keywords in English
Aedes aegypti
Conditional sterility
Population suppression
Transgenic
Abstract in English
The increasing number of infection cases by arboviruses, for instance Dengue, Zika and Chikungunya, it is necessary to develop new techniques to fight against these arboviruses. Genetic manipulation allowed the production of genetically modified mosquitoes that are capable of suppressing the wild population or prevent pathogens transmission and avoid disease development. The project proposes to establish lines for population suppression of Aedes aegypti, one of the main vectors of those diseases. This construction presents the conditional sterility due to the presence or absence of antibiotics in the environment where these mosquitoes are developed during the larval stage. Thus, without the need to use radiation for sterile insects, it is possible to improve the quality of the released adult males and enhance the competitiveness thereof in competing with wild females and additionally generate population suppression.
 
WARNING - Viewing this document is conditioned on your acceptance of the following terms of use:
This document is only for private use for research and teaching activities. Reproduction for commercial use is forbidden. This rights cover the whole data about this document as well as its contents. Any uses or copies of this document in whole or in part must include the author's name.
Publishing Date
2016-11-17
 
WARNING: The material described below relates to works resulting from this thesis or dissertation. The contents of these works are the author's responsibility.
  • Oliveira S de L, Carvalho DO, e CAPURRO, M L. Mosquito transgênico: do paper para a realidade [doi:10.7594/revbio.6b.08]. Revista da Biologia [online], 2011, vol. 6b, p. 38-43.
All rights of the thesis/dissertation are from the authors
CeTI-SC/STI
Digital Library of Theses and Dissertations of USP. Copyright © 2001-2021. All rights reserved.