Diversidade, taxonomia e filogenia de tripanossomatídeos da subfamilia Leishmaniinae.

Dominguez, Omar Ariel Espinosa

doi:10.11606/T.42.2016.tde-23122015-102417

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Doctoral Thesis

DOI

https://doi.org/10.11606/T.42.2016.tde-23122015-102417

Document

Doctoral Thesis

Author

Dominguez, Omar Ariel Espinosa (Catálogo USP)

Full name

Omar Ariel Espinosa Dominguez

Institute/School/College

Instituto de Ciências Biomédicas

Knowledge Area

Biology of Host-Pathogen Interactions

Date of Defense

2015-09-25

Published

São Paulo, 2015

Supervisor

Teixeira, Marta Maria Geraldes (Catálogo USP)

Committee

Teixeira, Marta Maria Geraldes (President)
Galati, Eunice Aparecida Bianchi
Mestriner, Clara Lúcia Barbieri
Shaw, Jeffrey Jon
Uliana, Silvia Reni Bortolin

Title in Portuguese

Diversidade, taxonomia e filogenia de tripanossomatídeos da subfamilia Leishmaniinae.

Keywords in Portuguese

Crithidia
Leishmania
Leptomonas
Endotrypanum
Filogenia
gGAPDH
ITSrDNA
Leishmaniinae
SSUrRNA

Abstract in Portuguese

A subfamília Leishmaniinae compreende espécies de tripanossomatídeos dos gêneros Crithidia, Leptomonas, Endotrypanum e Leishmania. O objetivo deste estudo foi caracterizar isolados da subfamília Leishmaniinae através de DNA Barcoding e inferir suas relações filogenéticas utilizando os genes SSUrDNA, gGAPDH, CATB, HSP70 e ITS1rDNA como marcadores genéticos. As espécies estudadas foram segregadas em dois clados principais, um formado por Endotrypanum-Leishmania e genótipos de L. costarricensis posicionados como clado basal. E outro clado que contém espécies monoxênicas as quais foram separados em diferentes subclados (Crithidia, Leptomonas, Lotmaria, Termófilo e três subclados homogêneos representados por C. inconstans, C. acanthocephali e isolados de Rondônia). No gênero Leishmania as análises posicionaram as espécies pouco estudadas do complexo L. enriettii como o grupo mais basal. Os isolados de lagartos Moçambique formaram um grupo monofilético com as espécies do subgênero L. (Sauroleishmania), corroborando, também, sua relação com o subgênero L. (Leishmania).

Title in English

Diversity, taxonomy and phylogeny of trypanosomatids of Leishmaniinae subfamily.

Keywords in English

Crithidia
Leishmania
Leptomonas
Endotrypanum
gGAPDH
ITSrDNA
Leishmaniinae
Phylogeny
SSUrRNA

Abstract in English

The Leishmaniinae subfamily comprises trypanosomatid species from genera Crithidia, Leptomonas, Endotrypanum and Leishmania. The aim of this study was to characterize Leishmaniinae isolates through DNA Barcoding and further infer their phylogenetic relationships using SSUrDNA, gGAPDH, CATB, HSP70 and ITS1rDNA genes as genetic markers. The Leishmaniinae species was segregated into two major clades, one formed by Endotrypanum-Leishmania with L. costarricensis genotypes resolving as a basal clade. The other clade contained the monoxenic species which were separated in different subclades (Crithidia, Leptomonas, Lotmaria, Termofilo, and three homogeneous subclades represented by C. inconstans, C. acanthocephali and Rondônia isolates). In Leishmania genus analyzes positioned the poorly investigated species of the complex L. enriettii as the most basal group. Isolates from Mozambique lizards formed a monophyletic group with the species of the subgenus L. (Sauroleishmania), was corroborated its relationship to the subgenus L. (Leishmania) too.

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Publishing Date

2016-03-15

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