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Doctoral Thesis
DOI
10.11606/T.42.2010.tde-14012011-125021
Document
Author
Full name
Renata Gonçalves Dias
Institute/School/College
Knowledge Area
Date of Defense
Published
São Paulo, 2010
Supervisor
Committee
Cury, Yara (President)
Chacur, Marucia
Costa, Soraia Kátia Pereira
Mello, Suzana Beatriz Verissimo de
Pagano, Rosana de Lima
Title in Portuguese
Caracterização da inflamação articular induzida por fosfolipase A2 - grupo II A: determinação das alterações histopatológicas, comportamentais e mediação química.
Keywords in Portuguese
Bothrops asper
Artrite animal
Dor
Fosfolipases
Inflamação articular
Miotoxina
Serpentes
Venenos de origem animal
Abstract in Portuguese
As fosfolipases A2 secretadas, particularmente do grupo II são abundantes nos venenos de serpentes, incluindo o gênero Bothrops e estão envolvidas em diversos processos fisiológicos e fisiopatológicos, como inflamação e dor, incluindo artrite. Contudo, não está totalmente caracterizado o papel da FLA2 para a gênese e manutenção dos quadros de inflamação articular. Nosso objetivo foi padronizar um novo modelo de artrite, utilizando sFLA2 do grupo IIA (miotoxina II) isolada do veneno da serpente Bothrops asper e avaliar a mediação química envolvida no processo nociceptivo deste quadro. Os resultados indicaram aumento de permeabilidade vascular, infiltrado celular e hiperalgesia. A hiperalgesia é um processo multimediado com a participação de prostanóides, sendo sua produção decorrente da ativação de FLA2 endógenas. Estes dados sugerem que esta FLA2 pode se tornar uma ferramenta científica importante para o entendimento dos mecanismos fisiopatológicos envolvidos nos processos de inflamação articular.
Title in English
Characterization of joint inflammation induced by phospholipase A2 - group II: determination of the histopathological changes, behavioral and chemical mediation.
Keywords in English
Bothrops asper
Animal arthritis
Articular Inflammation
Myotoxin
Pain
Phospholipases
Poison of animal origin
Snakes
Abstract in English
Secretory phospholipases A2 are abundant in different animal tissues and particularly group II are found in venom snakes and are proteins involved in many physiological and pathophysiological processes, like inflammation and pain, components of arthritis. However, the involvement of PLA2 in the genesis and maintenance of articular inflammation is not well characterized. Our aim is to characterize the articular inflammatory response induced by Lys 49-PLA2 (IIA group) isolated from B. asper snake venom. It was analyzed the nociceptive process involved, developing a new experimental model of articular inflammation. Our results indicated that sPLA2 induces increase in the vascular permeability, cell migration and hyperalgesia. Hyperalgesia is a multimediated process and prostanoids are involved in the nociceptive process, being its production dependent of the endogenous PLA2 activation. These data indicate that this PLA2 could be an important scientific tool for the understanding of the pathophysiological mechanisms involved in articular inflammation processes.
 
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Publishing Date
2011-05-09
 
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