• JoomlaWorks Simple Image Rotator
  • JoomlaWorks Simple Image Rotator
  • JoomlaWorks Simple Image Rotator
  • JoomlaWorks Simple Image Rotator
  • JoomlaWorks Simple Image Rotator
  • JoomlaWorks Simple Image Rotator
  • JoomlaWorks Simple Image Rotator
  • JoomlaWorks Simple Image Rotator
  • JoomlaWorks Simple Image Rotator
  • JoomlaWorks Simple Image Rotator
 
  Bookmark and Share
 
 
Doctoral Thesis
DOI
10.11606/T.42.2011.tde-23012012-094328
Document
Author
Full name
Vanessa Olzon Zambelli
Institute/School/College
Knowledge Area
Date of Defense
Published
São Paulo, 2011
Supervisor
Committee
Cury, Yara (President)
Martins, Rodrigo Alvaro Brandão Lopes
Parada, Carlos Amilcar
Picolo, Gisele
Rae, Giles Alexander
Title in Portuguese
Avaliação da expressão e ativação de receptores opióides após injúria periférica em ratos.
Keywords in Portuguese
Alcalóides
Analgesia
Expressão gênica
Inflamação
Ópio
Ratos
Abstract in Portuguese
Este trabalho teve como objetivo avaliar os mecanismos envolvidos no aumento da eficácia analgesia da crotalfina (CRF), um peptídeo com atividade tipo opióide, na vigência de inflamação (prostaglandina E2/PGE2) ou lesão tecidual (constrição crônica do nervo isquiático/CCI). A PGE2 (intraplantar), em ratos, aumentou a expressão gênica e protéica de receptores opióides do tipo m e k, no gânglio da raiz dorsal (DRG) e nervo da pata (NP), quando comparado a animais naive. A CCI aumentou a expressão de receptores m e d, no DRG e diminuiu os níveis de receptores k. Embora a PGE2 e CCI, per se, não ativem receptores opióides o CRF e agonistas opióides acarretam maior ativação destes receptores na vigência de sensibilização por PGE2 e CCI. Ainda, o CRF ativa a via das MAPKs (ERK1/2 e JNK), apenas na presença de PGE2, efeito mediado por receptores do tipo k e PKCz. Estes dados mostram que a expressão e ativação de receptores opióides são diferentemente regulados pela injúria aguda ou crônica.
Title in English
Evaluation of expression and activation of opioid receptors after peripheral injury in rats.
Keywords in English
Alkaloids
Analgesia
Gene expression
Inflammation
Opium
Rats
Abstract in English
This study aimed to evaluate the mechanisms involved in the increased analgesic efficacy of crotalphine (CRP), an opioid-like peptide, under inflammation prostaglandin E2 (PGE2) and tissue injury (chronic constriction injury/CCI). PGE2 (intraplantar) in rats, increases the genic and proteic expression of m- and k-opioid receptors in the dorsal root ganglia (DRG) and nerve paw (NP), when compared to naïve rats. CCI up-regulates the expression of m and d -opioid receptors in DRG and NP. In contrast, k-opioid receptors were down-regulated by CCI. Although PGE2 and CCI, per se, do not activate opioid receptors, CRP and opioid agonists lead to a higher activation of these receptors in NP slices under PGE2 or CCI sensitization or in DRG cells incubated with PGE2. Moreover, CRP activates ERK1/2 and JNK MAPKs pathways, k-receptor and PKCz-mediated, only when the cells were pre-incubated with PGE2. These data indicate that the expression and activation of opioid receptors are distinctly regulated by the presence of acute or chronic injury.
 
WARNING - Viewing this document is conditioned on your acceptance of the following terms of use:
This document is only for private use for research and teaching activities. Reproduction for commercial use is forbidden. This rights cover the whole data about this document as well as its contents. Any uses or copies of this document in whole or in part must include the author's name.
Publishing Date
2012-03-16
 
WARNING: Learn what derived works are clicking here.
All rights of the thesis/dissertation are from the authors
Centro de Informática de São Carlos
Digital Library of Theses and Dissertations of USP. Copyright © 2001-2021. All rights reserved.