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Master's Dissertation
DOI
10.11606/D.42.2008.tde-03062008-135402
Document
Author
Full name
Darine Christina Maia Villela
Institute/School/College
Knowledge Area
Date of Defense
Published
São Paulo, 2008
Supervisor
Committee
Afeche, Solange Castro (President)
Ferreira, Ana Carolina Franco
Scavone, Cristoforo
Title in Portuguese
Síntese de melatonina na glândula pineal de ratos: modulação pelo glutamato.
Keywords in Portuguese
Astrócitos
Glândula pineal
Glutamato
Melatonina
NF-kB
Pinealócitos
Abstract in Portuguese
Esse trabalho teve como objetivo estudar os efeitos do glutamato sobre a síntese de melatonina, avaliando a participação dos diferentes tipos de receptores e uma possível interação entre pinealócitos e astrócitos; além de avaliar o papel do fator de transcrição NF-kB nos efeitos modulatórios do glutamato. Para tanto, glândulas pineais de ratos foram mantidas em cultura e estimuladas com diferentes concentrações de glutamato e também com diversos agonistas glutamatérgicos. Culturas de pinealócitos isolados e co-culturas de pinealócitos e astrócitos também foram usadas. A melatonina foi quantificada em HPLC com detecção eletroquímica. Para caracterização dos receptores de glutamato foi feita uma análise de RT-PCR, e a ativação do NF-kB foi avaliada por ensaio de gel de retardo. Os resultados mostram que o glutamato exerce um efeito inibitório sobre a síntese de melatonina através de uma ação sobre os receptores metabotrópicos dos grupos I e II e ionotrópico do tipo AMPA e que as ações do glutamato são mediadas pela ativação do NF-kB dos astrócitos.
Title in English
Glutamate modulation of melatonin synthesis in the rat pineal gland.
Keywords in English
Astrocytes
Glutamate
Melatonin
NF-kB
Pineal gland
Pinealocytes
Abstract in English
The aim of this work was to study the effects of glutamate on melatonin synthesis, investigating the glutamate receptors involved and a possible interaction between the two predominant cell types of the pineal gland, pinealocytes and astrocytes; moreover, it was investigated the involvement of the transcription factor NF-kB on the glutamate effects. To accomplish this, pineal glands were kept in culture and stimulated with glutamate or glutamate agonists. Isolated pinealocytes or in association with astrocytes in culture were also stimulated with glutamate. Melatonin was quantified by HPLC with electrochemical detection. Glutamate receptors were characterized by RT-PCR and NF-kB activation was evaluated by gel shift assay. The data showed that glutamate has an inhibitory effect on melatonin synthesis that is mediated by groups I and II glutamate metabotropic receptors and AMPA receptor and that this effect involves the activation of astrocytic NF-kB.
 
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Publishing Date
2008-06-11
 
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