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Master's Dissertation
DOI
10.11606/D.42.2016.tde-09112016-151614
Document
Author
Full name
Daniel Blanc Araujo
Institute/School/College
Knowledge Area
Date of Defense
Published
São Paulo, 2016
Supervisor
Committee
Abdulkader, Fernando Rodrigues de Moraes (President)
Cassola, Antonio Carlos
Miranda Filho, Manoel de Arcisio
Title in Portuguese
O transporte de ânions em células INS-1E não compõe parte do mecanismo da via de amplificação da secreção de insulina estimulada pela glicose.
Keywords in Portuguese
Amplificação
Ânion
Glicose
INS-1E
Insulina (secreção)
Transporte
Abstract in Portuguese
A via de amplificação da secreção de insulina estimulada por glicose (GSIS) é um fenômeno discutido na literatura, cujos componentes são amplamente debatidos. Evidências sugerem que a condutância a Cl- compõe parte desta via. Porém, o mecanismo pelo qual essa condutância desempenharia papel na via de amplificação ainda é debatido, e, além disso, as ferramentas farmacológicas para estudo dessas afeta o transporte de outros ânions, como bicarbonato (HCO3-). Buscamos neste trabalho compreender a contribuição do transporte desses ânions para a via de amplificação da GSIS levando em consideração a distribuição de Cl- e HCO3- extracelular em células INS-1E. Concluímos que o transporte de ânions nas células INS-1E não contribui para a via de amplificação da GSIS, porém essas células não expressaram os canais CFTR e Anoctamina 1 que foram relacionados com esse fenômeno. Acreditamos que em células secretoras de insulina que expressem esses canais, o transporte de ânions possua alguma relevância funcional.
Title in English
The anion transport in INS-1E cell line do not composes part of the mechanism of the amplification pathway of glucose stimulated insulin secretion.
Keywords in English
Amplifying
Anion
Glucose
INS-1E
Insulin (secretion)
Transport
Abstract in English
The amplification pathway of glucose stimulated insulin secretion (GSIS) is a phenomenon discussed in the literature, which components are broadly debated. Evidence suggests that Cl- conductance composes part of this pathway. However, the mechanism that this conductance would play role on the amplification pathway still is debated, and, besides that, the pharmacological tools to study these affects transport of other anions, such as bicarbonate (HCO3-). We aimed in this study to understand the contribuition of anion transport for the amplification of GSIS considering the Cl- and HCO3- extracellular distribuition in INS-1E cells. We concluded that anion transport in INS-1E cell line do not contribute for the amplification pathway of GSIS, however those cells do not express CFTR and Anoctamin 1 channels which were related with this phenomenon. We believe that in insulin secretin cells that express those channels, the anion transport may have a functional relevance.
 
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Release Date
2018-11-09
Publishing Date
2016-11-09
 
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