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Doctoral Thesis
DOI
10.11606/T.42.2012.tde-25072012-095638
Document
Author
Full name
Gisele Kruger Couto
Institute/School/College
Knowledge Area
Date of Defense
Published
São Paulo, 2012
Supervisor
Committee
Rossoni, Luciana Venturini (President)
Fortes, Zuleica Bruno
Lemos, Virgínia Soares
Oliveira, Edilamar Menezes de
Stefanon, Ivanita
Title in Portuguese
Estudo da função em artérias de resistência de ratos pós-infarto do miocárdio.
Keywords in Portuguese
Artéria coronária
Artéria de resistência do músculo esquelético
Infarto do miocárdio
Óxido nítrico
Ratos
Reatividade vascular
Abstract in Portuguese
Esta tese avaliou a função em artérias de resistência do músculo esquelético (ARME) e em coronárias septais (CS) de ratos com disfunção ventricular esquerda leve (LDV) e severa (SDV) pós-infarto do miocárdio (IM). As ARME e as CS foram montadas em miógrafo de arame. O relaxamento dependente do endotélio, mediado pela acetilcolina (ACh), foi reduzido nas ARME dos SDV e preservado nos LDV. A resposta à ACh foi reduzida na CS dos SDV e aumentada nos LDV. O relaxamento mediado pelo doador de óxido nítrico (NO) foi preservado em todas as artérias. Utilizando-se a sonda DAF-2 em cortes de CS, antes e após estimulação com ACh, detectou-se menor produção de NO nos SDV e maior produção de NO nos LDV. Na CS observou-se aumento das espécies reativas de oxigênio nos SDV e redução destas nos LDV. Detectou-se maior expressão protéica da sintase de NO endotelial e neuronal e das isoformas da superóxido dismutase na CS dos LDV em relação ao SHAM. Conclui-se que a função nas ARME e nas CS é modulada diferentemente na dependência do estado da função cardíaca pós-IM.
Title in English
Vascular resistance function in myocardial infarction rats.
Keywords in English
Coronary artery
Myocardial infarction
Nitric oxide
Rats
Skeletal muscle resistance arteries
Vascular reactivity
Abstract in English
This study assessed the reactivity in skeletal muscle resistance arteries (SMRA) and in septal coronary arteries (SC) from rats with slight (sliLVD) and severe (sevLVD) left ventricular dysfunction after myocardial infarction (MI). SMRA and the SC were mounted on a wire myograph. The endothelium-dependent relaxation by acetylcholine (ACh) was reduced in SMRA from sevLVD; but it was preserved in sliLVD. The ACh-relaxation was reduced in SC from sevLVD; while it was increased in sliLVD. The nitric oxide (NO) donor relaxation was unchanged in all arteries. Using DAF-2 probe in SC, before and after ACh stimulation, it was observed NO production decreased in SC from sevLVD, while it was increased in sliLVD. Reactive oxygen species was increased in SC from sevLVD and it was reduced in sliLVD. Neuronal and endothelial NO synthase and superoxide dismutase isoforms protein expression were greater in SC from sliLVD as compared to SHAM. In conclusion, SMRA and SC function is differentially modulated on the dependency of the cardiac function after MI.
 
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Publishing Date
2012-08-15
 
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