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Doctoral Thesis
DOI
10.11606/T.42.2012.tde-29012013-092713
Document
Author
Full name
Ana Bárbara Teixeira Alves Wagner
Institute/School/College
Knowledge Area
Date of Defense
Published
São Paulo, 2012
Supervisor
Committee
Machado, Ubiratan Fabres (President)
Christoffolete, Marcelo Augusto
Festuccia, William Tadeu Lara
Giannocco, Gisele
Queiroz, Márcia Silva
Title in Portuguese
Efeito da associação entre hipertensão arterial e diabetes na expressão do Slc2a4/GLUT4 no músculo esquelético, provável participação do sistema nervoso autonômo simpático b-adrenérgico.
Keywords in Portuguese
Diabetes mellitus
Hipertensão
Músculo esquelético
Neuropatias diabéticas
Receptores adrenérgicos
Sistema nervoso simpático
Abstract in Portuguese
Investigamos como a atividade simpática atua na expressão do Slc2a4/GLUT4 em músculo esquelético de ratos normotensos (Wistar) e espontâneamente hipertensos (SHR) diabéticos, tratados com salina, insulina, propranolol e propranolol+insulina. A insulina reduziu a glicemia, glicosúria e volume urinário, aumentou o peso e a expressão do Slc2a4 no sóleo, porém diminuiu a expressão do Slc2a4 no EDL. O propranolol reduziu a pressão caudal dos SHR, não alterou os parâmetros metabólicos, aumentou a expressão do Slc2a4 no EDL, mas reduziu no sóleo somente dos normotensos. O propranolol+insulina mostrou que em sóleo o efeito da insulina foi preponderante, entretanto, no EDL o propranolol atenuou o efeito da insulina. A expressão do Slc2a4 foi maior nos hipertensos (sóleo e EDL), sendo parcialmente acompanhada pela expressão da proteína, com algumas alterações pós-transcricionais ou por Tnfa. Portanto, o comprometimento da expressão do Slc2a4 induzido pelo diabetes é menor em SHR, provavelmente devido à hiper-atividade simpática presente no músculo esquelético.
Title in English
The effect of hypertension and diabetes association on Slc2a4/GLUT4 expression, possible b-adrenergic sympathectic nervous system participation.
Keywords in English
Adrenergic receptors
Diabetes mellitus
Diabetic neuropathies
Hypertension
Skeletal muscle
Sympathetic nervous system
Abstract in English
We investigated the participation of sympathetic activity in the regulation of Slc2a4/GLUT4 expression in skeletal muscle of diabetic Wistar and spontaneously hypertensive (SHR) rats, treated with saline, insulin, propranolol and propranolol+insulin. Insulin reduced glycemia, urinary volume and glucose, and increased body weight and Slc2a4 expression in soleus, but decreased it in EDL. Propranolol reduced arterial blood pressure in SHR, and did not alter the metabolic parameters, increased the mRNA expression in EDL, but reduced it in soleus of normotensive rats. Propranolol+insulin showed that in soleus the effect of insulin was preponderant, whereas in EDL propranolol attenuated the insulin effect. The Slc2a4 expression was higher in SHR (soleus, EDL), and it was similar to the protein expression, with some discrepancies, indicating post-transcription regulation, or Tnfa-related alteration. In conclusion, the impairment in Slc2a4 expression during diabetes is limited in SHR, probably because their high sympathetic activity in skeletal muscle.
 
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Release Date
2017-03-26
Publishing Date
2013-03-14
 
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