• JoomlaWorks Simple Image Rotator
  • JoomlaWorks Simple Image Rotator
  • JoomlaWorks Simple Image Rotator
  • JoomlaWorks Simple Image Rotator
  • JoomlaWorks Simple Image Rotator
  • JoomlaWorks Simple Image Rotator
  • JoomlaWorks Simple Image Rotator
  • JoomlaWorks Simple Image Rotator
  • JoomlaWorks Simple Image Rotator
  • JoomlaWorks Simple Image Rotator
 
  Bookmark and Share
 
 
Master's Dissertation
DOI
10.11606/D.43.2010.tde-14052010-111406
Document
Author
Full name
Aurea Beatriz Martins Bach
E-mail
Institute/School/College
Knowledge Area
Date of Defense
Published
São Paulo, 2010
Supervisor
Committee
Rabbani, Said Rahnamaye (President)
Bonagamba, Tito Jose
Figueiredo Neto, Antonio Martins
Title in Portuguese
Estudo da distrofia muscular em camundongos mdx com ressonância magnética nuclear
Keywords in Portuguese
Camundongos mdx
Distrofia muscular
Espectroscopia
Ressonância magnética nuclear
Abstract in Portuguese
Atualmente, a espectroscopia de Ressonância Magnética Nuclear (RMN) in vitro tem sido extensivamente empregada para estudar tecidos biológicos, atuando como uma poderosa ferramenta de análise química. Em particular, a RMN de próton (1H) e de fósforo (31P) vem sendo utilizada para estudar o metabolismo muscular de animais portadores de deficiências genéticas, como os camundongos com distrofia muscular mdx, modelos para a distrofia muscular Duchenne (DMD). A DMD, que afeta humanos, é um distúrbio recessivo ligado ao cromossomo-X e ocorre em 1 para cada 3500 nascidos vivos do sexo masculino. A DMD é caracterizada pela ausência da proteína distrofina, o que provoca um processo progressivo e rápido de degeneração muscular. Atualmente, o acompanhamento da evolução da doença e de benefícios de tratamentos é feito através de biópsias do tecido muscular. Neste estudo foram realizadas medidas de RMN de 1H em amostras de diafragma e do músculo quadríceps femural de camundongos mdx e de controle com 3 e 6 meses de idade. Os resultados foram comparados com a análise histológica dos mesmos tecidos. O objetivo deste trabalho é monitorar o desenvolvimento normal dos músculos de animais de controle e o progresso da distrofia nos músculos de animais mdx, através da análise dos espectros de RMN. Foi possível identificar diferenças entre os grupos de animais a partir das integrais dos picos observados, mostrando que a distrofia acarreta alterações em diversas vias metabólicas nos camundongos mdx. Estes resultados formam a base para estudos da doença in vivo, para que então seja possível diferenciar músculos distróficos de músculos sadios e caracterizar diferentes estágios de evolução da doença de maneira não invasiva.
Title in English
Study of muscular dystrophy in mdx mice with nuclear magnetic resonance
Keywords in English
Mdx mice
Muscular dystrophy
Nuclear magnatic resonance
Spectroscopy
Abstract in English
Currently, Nuclear Magnetic Resonance spectroscopy in vitro has been extensively used to study biological tissues, acting as a powerful tool for chemical analysis. In particular, NMR of proton (1H) and phosphorus (31P) has been used to study muscle metabolism in animals with genetic diseases, such as mice with muscular dystrophy mdx, models for Duchenne muscular dystrophy (DMD). The DMD, which affects humans, is a recessive disorder linked to X-chromosome and occurs in 1 each 3,500 live births male. The DMD is characterized by the absence of dystrophin protein, which causes a progressive and rapid degeneration. Currently, the monitoring of disease progression and benefits of treatments is made by biopsy of muscle tissue. In this study, 1H NMR spectrum were acquired from samples of diaphragm and quadriceps muscle of mdx and control mice 3 or 6 months-old. Results were compared with histological analysis of the same tissues. The objective of this study is to monitor the normal development of the muscles of control animals and the progress of dystrophy in the muscles of mdx animals by analyzing the NMR spectra. Differences were found between the groups of animals comparing the integrals of the observed peaks, showing that dystrophy leads to alterations in several methabolic pathways in the mdx mouse. These results form the basis for studies of the disease in vivo, so then it can be possible to distinguish dystrophic muscles from healthy muscles and characterize different stages of the disease noninvasively.
 
WARNING - Viewing this document is conditioned on your acceptance of the following terms of use:
This document is only for private use for research and teaching activities. Reproduction for commercial use is forbidden. This rights cover the whole data about this document as well as its contents. Any uses or copies of this document in whole or in part must include the author's name.
Dissertacao.pdf (8.36 Mbytes)
Publishing Date
2010-05-19
 
WARNING: Learn what derived works are clicking here.
All rights of the thesis/dissertation are from the authors
Centro de Informática de São Carlos
Digital Library of Theses and Dissertations of USP. Copyright © 2001-2021. All rights reserved.