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Doctoral Thesis
DOI
10.11606/T.46.2018.tde-15102018-160730
Document
Author
Full name
Alcely Strutz Barroso
Institute/School/College
Knowledge Area
Date of Defense
Published
São Paulo, 2001
Supervisor
Committee
Colepicolo, Pio (President)
Abdalla, Dulcineia Saes Parra
Campa, Ana
Marques, Marcia Martins
Nicolau, Jose
Title in Portuguese
Modulação do estresse oxidativo em glândulas submandibulares de rato
Keywords in Portuguese
Biologia molecular
Bioquímica
Abstract in Portuguese
As espécies reativas de oxigênio geradas in vivo têm sido implicadas em vários mecanismos como apoptose, crescimento e diferenciação celular, câncer e inflamação. O estresse oxidativo é o produto do desbalanço entre os agentes prooxidantes e antioxidantes celulares, causando danos por meio da peroxidação lipídica, oxidação de proteínas e de DNA. Neste trabalho avaliou-se o efeito do estresse oxidativo em glândulas submandibulares de rato e em células acinares dispersas tratadas com isoproterenol (ISO), cicloheximida (CHX), carbacol (CA) e propanolol (PROP). Observou-se aumento dos níveis de MDA em homogenados de glândulas de ratos tratados com ISO e CHX e em células acinares dispersas incubadas com ISO e CHX. Tratamentos semelhantes causaram aumento na oxidação protéica em cultura de células, mas o mesmo aumento não ocorreu in vivo. Em relação aos agentes antioxidantes, observou-se um aumento no poder redutor (conteúdo de GSH) em homogenados de glândulas submandibulares tratadas com ISO e em células dispersas incubadas com ISO. A atividade de superóxido dismutase (SOD) também aumentou em tratamentos com ISO in vivo e os "Western Blots" mostraram uma variação acentuada nos níveis protéicos da isoforma MnSOD. A atividade de SOD, induzida por ISO em células dispersas, não ocorre quando as células foram pré-incubadas com PROP, o que sugere a ativação da atividade de SOD via receptores β-adrenérgicos.
Title in English
Modulation of oxidative stress in submandibular mouse glands
Keywords in English
Biochemistry
Molecular biology
Abstract in English
Reactive oxygen species (ROS) have been implicated in many mechanisms as apoptosis, cell growth and differentiation, cancer and inflammation. Oxidative stress is the product of the imbalance of the prooxidants and antioxidants agents within the cell and it has been shown to cause damage by producing peroxidation of fatty acid and oxidation of proteins and DNA. In this work the oxidative stress was evaluated on rat submandibular glands and on dispersed acinar cells treated with isoproterenol (ISO), cycloheximide (CHX), carbachol (CA) and propanolol (PROP). The level of lipid peroxidation on rats submandibular glands and on dispersed acinar cells were increased by ISO and CHX treatment. Protein oxidation level was increased on cell culture treated with ISO, but the same results was not found in vivo treatments. Regards the antioxidant agents, the GSH content was found to increase on both in vivo and on cell culture by ISO treatment. The superoxide dismutase (SOD) activity in vivo increased and the Western Blots showed a significant MnSOD variation level. The SOD was induced by ISO treatment on cell culture, but the same increasing was not found with previous cells treatment with PROP. This result suggest the involvement of a β-adrenergic receptor on SOD activity.
 
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Publishing Date
2018-10-15
 
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