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Master's Dissertation
DOI
10.11606/D.46.2010.tde-13122010-102634
Document
Author
Full name
Guilherme Lopes Batista
E-mail
Institute/School/College
Knowledge Area
Date of Defense
Published
São Paulo, 2010
Supervisor
Committee
Gutz, Ivano Gebhardt Rolf (President)
Cardoso, Arnaldo Alves
Rothschild, Lilian
Title in Portuguese
Análise da acetona em ar exalado: metodologia para estudo em pacientes hospitalizados
Keywords in Portuguese
Acetona
Amostragem de gases
Análise de ar exalado
Biomarcadores
Espectrofotometria
Método não-invasivo
Pacientes internados (Análise química)
Química anatítica
Abstract in Portuguese
A dissertação se situa no campo de pesquisa de métodos não invasivos de análise clínica, mais especificamente, de prognóstico da gravidade de insuficiência cardíaca, IC, com base em bioindicador presente no ar exalado por pacientes, em estudo no IncorHCFMUSP. O bioindicador mais relevante encontrado por GC-MS foi a acetona (propanona). Para a coleta do ar exalado desenvolveu-se dispositivo que compreende frasco borbulhador (impinger) com difusor, contendo 5 mL de água, imerso em banho de gelo e acoplado a saco plástico para definição de volume amostrado (7,6 L). A preservação de amostras deu-se por congelamento a -80ºC. Para a determinação do analito, escolheu-se método espectrofotométrico (474 nm) baseado na reação da acetona com salicilaldeído em meio básico, apresentando limite de detecção de 0,3 mg/L em fase líquida e 0,3 µg/L em ar exalado. Foram realizados estudos de eficiência de coleta por geração de atmosfera controlada. Resultados iniciais obtidos com amostras coletadas de pacientes portadores de IC pelos colaboradores do Incor indicam a potencialidade do bioindicador e dos equipamentos e métodos desenvolvidos para acetona nesta dissertação.
Title in English
Breath Acetone Analysis: Methodology for studying hospitalized pacients
Keywords in English
Acetone
Analytical chemistry
Breath Analysis
Gas Sampling
Non- Invasive analysis
Spectrophotometry
Abstract in English
The dissertation fits in the research field of noninvasive clinical analysis, more specifically, the prognosis of the severity of heart failure, HF, based on a biomarker found in the air exhaled by patients, in a study at InCor-HCFMUSP. The most relevant biomarker found by GC-MS was acetone (propanone). For the breath collection a device was developed which comprises a scrubber flask (impinger) with diffuser, containing 5 mL of water, immersed in an ice bath and attached to a plastic bag for delimitation of the sample volume (7,6 L). For the determination of acetone a spectrometric method (474 nm) was chosen based on a reaction of acetone with salicylaldehyde in alkaline medium, with detection limits of 0,3 mg/L in liquid phase and 0,3 µg/L in breath. Preliminary studies made with samples collected from HF patients by the co-workers from InCor indicate the potential of the identified biomarker and the suitability of the equipment and methods developed in this dissertation for its analysis.
 
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Publishing Date
2011-04-04
 
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