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Doctoral Thesis
DOI
10.11606/T.5.2006.tde-12042006-085305
Document
Author
Full name
Andrea Glezer
Institute/School/College
Knowledge Area
Date of Defense
Published
São Paulo, 2005
Supervisor
Committee
Bronstein, Marcello Delano (President)
Abucham Filho, Júlio Zaki
Bartolini, Paolo
Latrônico, Ana Claudia
Vieira, José Gilberto Henriques
Title in Portuguese
"Estudo da atividade biológica da macroprolactina humana em células Nb2 e em células Ba/F-03 transfectadas com o receptor de prolactina humano forma longa"
Keywords in Portuguese
Bioensaios/métodos
Hiperprolactinemia
Isoformas de proteínas
Prolactina
Prolactinoma
Receptores da prolactina
Abstract in Portuguese
A macroprolactinemia é condição freqüente na hiperprolactinemia e em geral, sem impacto clínico. Os dados sobre a atividade biológica da macroprolactina (bbPRL) são controversos e baseados em bioensaio heterólogo com células de rato Nb2. A atividade biológica da bbPRL é observada in vitro e não in vivo, provavelmente porque seu alto peso molecular evita sua passagem pelos capilares. A bioatividade da bbPRL talvez varie de acordo com a especificidade do receptor de prolactina (PRLR). Avaliamos a bioatividade da bbPRL de indivíduos macroprolactinêmicos (Grupo I, n = 18) e da PRL monomérica (mPRL) de pacientes hiperprolactinêmicos sem bbPRL (Grupo II, n = 5) em Nb2 e em células Ba/F-LLP, transfectadas com o PRLR humano. Enquanto ambos ensaios apresentam resultados similares para a atividade de mPRL, nossos resultados indicam que a atividade da bbPRL é presente em ensaio heterólogo e não em ensaio homólogo. O ensaio Ba/F-LLP é sensível e apresenta melhor correlação com a atividade in vivo da bbPRL
Title in English
Human macroprolactin biological activity study in Nb2 cells and in Ba/F-03 cells expressing human long prolactin receptor
Keywords in English
Biological assay/methods
Hyperprolactinemia
Prolactin
Prolactinoma
Protein Isoforms
Receptors Prolactin
Abstract in English
Macroprolactinemia is a frequent finding in hyperprolactinemic individuals, usually without clinical impact. Data on biological activity of macroprolactin (bbPRL) is mostly based on a heterologous bioassay (Nb2 cell). Biological activity of bbPRL observed in vitro but not in vivo maybe due to its high molecular weight preventing its passage through capillary barrier. Alternatively, bbPRL bioactivity may differ depending on the PRL receptor species specificity. BbPRL from macroprolactinemic individuals and monomeric PRL (mPRL) from hyperprolactinemic patients without macroprolactinemia were tested in two bioassays: Nb2 and in Ba/F-LLP, which expresses human prolactin receptor. While both bioassays achieve similar results considering mPRL activity, our results indicate that bbPRL displays activity in a heterologous but not in a homologous bioassay, consistently with the apparent absence of bbPRL bioactivity in vivo
 
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AndreaGlezer.pdf (1.10 Mbytes)
Publishing Date
2006-04-12
 
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