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Master's Dissertation
DOI
10.11606/D.5.2005.tde-19102005-153641
Document
Author
Full name
Suzete Cleusa Ferreira
Institute/School/College
Knowledge Area
Date of Defense
Published
São Paulo, 2005
Supervisor
Committee
Sabino, Ester Cerdeira (President)
Buccheri, Valeria
Kallas, Esper Georges
Title in Portuguese
"Prevalência do HHV - 8 em doadores de sangue e politransfundidos na cidade de São Paulo, pelas técnicas de imunofluorescência indireta e Nested PCR"
Keywords in Portuguese
DOADORES DE SANGUE
HERPESVIRUS HUMANO 8
REAÇÃO EM CADEIA DA POLIMERASE
TRANSFUSÃO INDIRETA DE FLUORESCÊNCIA PARA ANTICORPO
Abstract in Portuguese
A prevalência de anticorpos e DNA viral de HHV-8 em 400 doadores de sangue e 50 indivíduos politransfundidos da Fundação Pró-Sangue Hemocentro de São Paulo, através das técnicas de Nested PCR e IFI para antígenos lítico e latente. Foi detectada uma soroprevalência mais alta entre doadores de sangue(4%) quando comparados a indivíduos politransfundidos (14%) (p= 0,002). A prevalência também foi mais alta entre mulheres em relação a homens, 7% versus 1.8% (p= 0,012; 95% IC 1,14 - 16,6). Foram detectados DNA de HHV-8 no plasma e PBMC de um doador 0.25%. Esta amostra foi seqüenciada confirmando a presença de HHV-8. O encontro de DNA viral no plasma de um doador de sangue indica a transfusão sanguínea como um possível meio de transmissão deste agente
Title in English
Prevalence of HHV-8 in blood donors and recipients in the city of São Paulo, by indirect imunofluorescent assay and Nested PCR
Keywords in English
BlOOD DONORS
BLOOD TRANSFUSION
CROSS-SECTIONAL STUDIES
FLUORESCENT ANTIBODY TECHNIQUE INDIRECT
HERPESVIRUS 8 HUMAN
POLYMERASE CHAIN REACTION
Abstract in English
The prevalence of antibodies and DNA viral of HHV-8 in 400 donors of blood and 50 receiving of the Fundação Pró-Sangue Hemocentro of São Paulo, through Nested PCR'S techniques and IFI for lithic and latent antigens. A seroprevalence of 4% was detected in the donors of blood and 14% in receiving, significantly high in comparison with the prevalence in donors of blood (p = 0,002). There was a significant difference in the prevalence among men 1,8% and 7% in the women (p= 0,012; 95% CI 1,14 - 16,6). They were detected DNA of HHV-8 in the plasma and a donor's 6,3% PBMC, and it was sequenced and submitted to the Gene Bank where presented 100% of identity. This allows to define the transmission tax and the need of introducing methods for this agent's selection
 
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10Resultados.pdf (32.29 Kbytes)
11discussao.pdf (9.94 Kbytes)
12conclusao.pdf (8.49 Kbytes)
13anexos.pdf (14.99 Kbytes)
14referencias.pdf (80.36 Kbytes)
1capa.pdf (4.64 Kbytes)
3sumario.pdf (15.70 Kbytes)
4resumo.pdf (5.90 Kbytes)
5summary.pdf (5.90 Kbytes)
6introducao.pdf (576.11 Kbytes)
7justificativa.pdf (5.95 Kbytes)
8objetivo.pdf (5.63 Kbytes)
9materiaismetodos.pdf (246.81 Kbytes)
Publishing Date
2005-10-20
 
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