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Master's Dissertation
DOI
10.11606/D.5.2005.tde-13042006-115452
Document
Author
Full name
Luciana de Freitas Velloso Monte
Institute/School/College
Knowledge Area
Date of Defense
Published
São Paulo, 2005
Supervisor
Committee
Ramos, Sonia Regina Testa da Silva (President)
Levi, José Eduardo
Okay, Thelma Suely
Title in Portuguese
"Detecção de Staphylococcus aureus resistente à meticilina por meio de multiplex PCR em amostras de secreção respiratória de pacientes com fibrose cística"
Keywords in Portuguese
Fibrose cística
Infecções respiratórias/diagnóstico
Reação em cadeia por polimerase
Resistência à meticilina
Staphylococcus Aureus/isolamento & purificação
Abstract in Portuguese
S.aureus resistente à meticilina(SARM) é um problema em centros de fibrose cística(FC). Foi desenvolvido um multiplex PCR(mPCR) para detecção do SARM em secreção respiratória de 106 pacientes com FC. Foram usados 3 pares de primers para amplificar os genes: mecA, coa, 16S rRNA. O mPCR detectou até 0,25pg de DNA de SARM e identificou 70/106(66,0%) pacientes com S.aureus e 28/106(26,4%) com SARM. O mPCR mostrou especificidade, sensibilidade, valores preditivos positivo e negativo de 87,8%, 84,4%, 50% e 97,5%, considerando a cultura como padrão-ouro. Os resultados discordantes foram testados com outros primers, confirmando os obtidos pelo mPCR em 82/84. O mPCR mostrou-se método rápido e confiável para detecção de SARM
Title in English
Detection of methicillin-resistant Staphylococcus aureus by multiplex PCR in respiratory secretion of cystic fibrosis patients
Keywords in English
Cystic fibrosis
Methicillin resistance
Polymerase chain reaction
Respiratory tract infections/diagnosis
Staphylococcus aureus/isolation & purification
Abstract in English
Methicillin-resistant S.aureus(MRSA) is a significant concern in cystic fibrosis(CF) centers. A multiplex PCR(MPCR) was developed to detect MRSA in respiratory secretion of 106 CF patients. Three pairs of primers were used for amplification of genes: mecA, coa, 16S rRNA. MPCR detected 0.25pg of MRSA DNA and identified 70/106(66.0%) of patients with S.aureus and 28/106(26.4%) with MRSA. MPCR showed specificity, sensitivity, positive and negative predicted values at 87.8%, 84.4%, 50% and 97.5%, considering culture as the gold standard. Discrepant results were retested using different primers, and confirmed MPCR results in 82/84. The developed MPCR was found to be a rapid and reliable method for MRSA detection
 
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LucianaFVMonte.pdf (577.26 Kbytes)
Publishing Date
2006-04-17
 
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