• JoomlaWorks Simple Image Rotator
  • JoomlaWorks Simple Image Rotator
  • JoomlaWorks Simple Image Rotator
  • JoomlaWorks Simple Image Rotator
  • JoomlaWorks Simple Image Rotator
  • JoomlaWorks Simple Image Rotator
  • JoomlaWorks Simple Image Rotator
  • JoomlaWorks Simple Image Rotator
  • JoomlaWorks Simple Image Rotator
  • JoomlaWorks Simple Image Rotator
 
  Bookmark and Share
 
 
Mémoire de Maîtrise
DOI
10.11606/D.5.2017.tde-28112017-152103
Document
Auteur
Nom complet
Juliana Andrade Alcantara
Adresse Mail
Unité de l'USP
Domain de Connaissance
Date de Soutenance
Editeur
São Paulo, 2017
Directeur
Jury
Valente, Kette Dualibi Ramos (Président)
Bianchin, Marino Muxfeldt
Manreza, Maria Luiza Giraldes de
Sallet, Paulo Clemente
Titre en portugais
O estudo de polimorfismos da via dopaminérgica na epilepsia do lobo temporal causada por esclerose hipocampal
Mots-clés en portugais
Dopamina
Epilepsia do lobo temporal
Fator neurotrófico derivado do encéfalo
Hipocampo
Polimorfismo genético
Receptores dopaminérgicos
Resumé en portugais
Estudos clínicos nos pacientes com epilepsia mostram a importância da neurotransmissão modulada pela dopamina na epilepsia. Múltiplos fatores genéticos predispõem à epilepsia e há evidências de uma relação direta entre a epilepsia e as variações nos genes que codificam proteínas envolvidas na neurotransmissão dopaminérgica. O objetivo do nosso estudo foi investigar se os polimorfismos da via dopaminérgica e o Val66Met do BDNF estavam associados à ocorrência de epilepsia do lobo temporal causada por esclerose hipocampal. Para este fim, avaliamos 119 pacientes com epilepsia do lobo temporal causada por esclerose hipocampal e 113 voluntários saudáveis. Os participantes foram genotipados para os polimorfismos do gene DAT (3'UTR e Intron 8), receptores dopaminérgicos (DRD2 e DRD4), COMT, MAO e BDNF (Val66Met). Não houve diferença entre pacientes e controles para os polimorfismos relacionados ao DAT, Íntron 8 VNTR (p 0,395) e 3'UTR VNTR (p 0,614) e para a análise dos haplótipos (3'UTR e Intron 8) (p 0.205). Não houve diferença entre pacientes e controles para os polimorfismos dos receptores dopaminérgicos DRD2 rs1800497 (p 0.440), DRD4 rs1800955 (p 0.548) e DRD4 VNTR (p 0.318). Não observamos diferença entre pacientes e controles quanto aos polimorfismos COMT rs4680 (p 0.482) e MAOA_uVNTR (p 0.753), metabolizadores de DA. Não observamos diferença na distribuição genotípica do polimorfismo Val66Met (rs6265) do BDNF (p 0,636) e a distribuição alélica (p 0.471) no grupo de pacientes com epilepsia do lobo temporal causada por esclerose do hipocampo. Nossos achados demonstraram que os polimorfismos da via dopaminérgica e BDNF Val66Met analisados neste estudo não parecem estar associados à epilepsia de lobo temporal causada por esclerose de hipocampo
Titre en anglais
The study of dopaminergic pathway polymorphisms in temporal lobe epilepsy caused by hippocampal sclerosis
Mots-clés en anglais
Brain-derived neurotrophic factor
Dopamine
Epilepsy temporal lobe
Hippocampus
Polymorphism genetic
Receptors dopamine
Resumé en anglais
Clinical studies in patients with epilepsy showed the role of neurotransmission modulated by dopamine in epilepsy. Multiple genetic factors predispose to epilepsy; there is evidence for a direct relationship between epilepsy and variations in genes encoding proteins involved in dopaminergic neurotransmission. The aim of our study was to investigate if the polymorphism related to the dopaminergic pathway and BDNF polymorphism Val66Met were associated with the occurrence of temporal lobe epilepsy caused by hippocampal sclerosis. We assessed 119 patients with unequivocal temporal lobe epilepsy caused by hippocampal sclerosis and 113 healthy volunteers. Individuals were genotyped for DAT gene polymorphisms (3'UTR and Intron 8), dopaminergic receptors (DRD2 and DRD4), COMT, MAO and BDNF. There was no difference between patients and controls considering the polymorphisms related to DAT, Intron 8 VNTR (p 0,395) and 3'UTR VNTR (p 0.614) and for the analysis of haplotypes (3'UTR and Intron 8) (p 0.205). There was no difference between patients and controls considering the dopaminergic receptor polymorphisms DRD2 rs1800497 (p 0.440), DRD4 rs1800955 (p 0.548) and DRD4 VNTR (p 0.318). We observed no difference between patients and controls regarding COMT polymorphisms rs4680 (p 0.482) and MAOA_uVNTR (p 0.753), of dopaminergic metabolizers. We did not observe difference in the genotypic distribution of BDNF Val66Met polymorphism (rs6265) (p 0.636) and in the allelic distribution (p 0.4711) in the group with temporal lobe epilepsy caused by hippocampal sclerosis. Our findings suggest that the polymorphisms of the dopaminergic pathway evaluated in this study and BDNF Val66Me do not appear to be associated with temporal lobe epilepsy caused by hippocampal sclerosis
 
AVERTISSEMENT - Regarde ce document est soumise à votre acceptation des conditions d'utilisation suivantes:
Ce document est uniquement à des fins privées pour la recherche et l'enseignement. Reproduction à des fins commerciales est interdite. Cette droits couvrent l'ensemble des données sur ce document ainsi que son contenu. Toute utilisation ou de copie de ce document, en totalité ou en partie, doit inclure le nom de l'auteur.
Date de Publication
2017-11-29
 
AVERTISSEMENT: Apprenez ce que sont des œvres dérivées cliquant ici.
Tous droits de la thèse/dissertation appartiennent aux auteurs
CeTI-SC/STI
Bibliothèque Numérique de Thèses et Mémoires de l'USP. Copyright © 2001-2019. Tous droits réservés.