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Doctoral Thesis
DOI
https://doi.org/10.11606/T.5.2020.tde-20032020-141735
Document
Author
Full name
Daniel Lorena Dutra
E-mail
Institute/School/College
Knowledge Area
Date of Defense
Published
São Paulo, 2019
Supervisor
Committee
Voegels, Richard Louis (President)
Mion, Olavo de Godoy
Nakanishi, Marcio
Pinna, Fabio de Rezende
Title in Portuguese
Composição e diversidade do microbioma do meato médio de pacientes com rinossinusite crônica com polipose nasal
Keywords in Portuguese
Análise de sequência de DNA
Microbioma
Pólipos nasais
RNA ribossômico 16S
Seio etmoidal
Sequenciamento de nucleotídeos em larga escala
Sinusite
Abstract in Portuguese
Introdução: A comunidade microbiológica presente no corpo humano apresenta papel crucial na homeostase normal de diversos sistemas fisiológicos e em inúmeros cenários fisiopatológicos. O surgimento de tecnologias que independem de cultura está permitindo o mapeando desta microbiota com alta sensibilidade. Nos seios paranasais saudáveis e na rinossinusite crônica esta comunidade ainda precisa ser determinada, não há estabelecido um microbioma nasossinusal típico. Objetivo: O objetivo deste estudo é avaliar a composição e diversidade do microbioma do meato médio de pacientes brasileiros adultos com rinossinusite crônica com polipose, utilizando tecnologia de sequenciamento de nova geração. Método: Realizou-se coleta com swab de meato médio de 20 pacientes saudáveis, considerado grupo controle e 29 pacientes com rinossinusite crônica com polipose (RSCcp). Após extração de DNA da amostra coletada, procedeu-se o sequenciamento de regiões variáveis V3-V4 do gene 16S rRNA para identificação filogenética bacteriana. A composição foi determinada por meio das abundâncias relativas dos gêneros mais prevalentes. Avaliação da diversidade foi realizada utilizando os índices mais frequentes. Resultados: Os gêneros com maior abundância relativa nos controles foram Corynebacterium, Staphylococcus e Streptococcus. Na RSCcp os gêneros mais abundantes foram Staphylococcus, Haemophilus e Corynebacterium, mas com depleção significativa dos representantes das Corynebacteriaceae. Os índices de diversidade e a riqueza não apresentaram diferença estatisticamente significativa entre os 2 grupos. Conclusão: Os gêneros Staphylococcus, Haemophilus e Corynebacterium, apresentaram dominância na composição do meato médio de pacientes com RSCcp. A comunidade bacteriana deste sítio anatômico mostrou uma depleção significativa de Corynebacterium
Title in English
Middle meatus microbiome composition and diversity of patients with chronic rhinosinusitis with nasal polyps
Keywords in English
16S RNA
DNA sequencing
Ethmoid sinus
Microbiome
Nasal polyps
Next-generation sequencing
Sinusitis
Abstract in English
Introduction: The microbiological community present in the human body plays a crucial role in the normal homeostasis of several physiological systems and numerous pathophysiological scenarios. The emergence of culture-independent technologies is allowing the mapping of this microbiota with high sensitivity. In healthy paranasal sinuses and chronic rhinosinusitis, this community is still being determined. The core sinonasal microbiome still needs to be established. Objective: This study aims to evaluate the middle meatus microbiome composition of adult Brazilian patients with chronic rhinosinusitis with polyps using next-generation sequencing technology. Methods: Middle meatus swabs from 20 healthy subjects and 29 patients with chronic rhinosinusitis with polyps (CRSwNP) were collected. Extraction of DNA from samples was performed and phylogenetic identification was determined using 16S rRNA gene sequencing of V3-V4 variable regions. The composition was determined through relative abundances of the most prevalent genera. Diversity was evaluated using the most common diversity indexes. Results: The genera with the highest relative abundances in the controls were Corynebacterium, Staphylococcus, and Streptococcus. In CRSwNP the most abundant genera were Staphylococcus, Haemophilus Corynebacterium, but with significant depletion of Corynebacteriaceae representatives. Diversity and richness did not reach a statistically significant difference between the 2 groups. Conclusion: The genera Staphylococcus, Haemophilus and Corynebacterium were dominant in the middle meatus composition of Brazilian adult patients with CRSwNP. The bacterial community of this anatomical site showed a significant depletion of Corynebacterium
 
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Publishing Date
2020-03-20
 
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