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Mémoire de Maîtrise
DOI
10.11606/D.5.2005.tde-29092005-123131
Document
Auteur
Nom complet
Clarice Pires Abrantes Lemos
Adresse Mail
Unité de l'USP
Domain de Connaissance
Date de Soutenance
Editeur
São Paulo, 2005
Directeur
Jury
Cançado, Eduardo Luiz Rachid (Président)
Gonçalves, Luciana Lofêgo
Oliveira, Claudia Pinto Marques Souza de
Titre en portugais
"Pesquisa do anticorpo antitransglutaminase tissular avaliando as interações da transglutaminase com a fibronectina e comparação com os resultados de dois ensaios comerciais"
Mots-clés en portugais
DOENÇA CELÍACA/diagnóstico
ELISA
FIBRONECTINAS
TRANSGLUTAMINASE
Resumé en portugais
Os objetivos desse estudo foram: 1) Padronizar a pesquisa do anti-tTg, comparando-o com o anticorpo antiendomísio (AAE) e 2) Avaliar as interações da tTg com a fibronectina. 49 celíacos e 124 controles com AAE negativo foram avaliados. O AAE foi pesquisado por imunofluorescência indireta e a reatividade contra a tTg e a fibronectina por ELISA in house e com kits comerciais. O antitTg foi positivo em 46,9% e 100% dos celíacos com o ELISA in house e com kits comerciais, respectivamente. A adição de fibronectina não melhorou a sensibilidade do ELISA. Em conclusão: a detecção do antitTg por ELISA apresenta percentual elevado de falso-positivos, não podendo substituir a pesquisa do AAE
Titre en anglais
Standardization of anti-tissue transglutaminase antibody detection and assessment of transglutaminase interactions with fibronectin : comparison of the results with two commercially available essays
Mots-clés en anglais
CELIAC DISEASE/diagnosis
ENZYME-LINKED IMMUNOSORBENT ASSAY
FIBRONECTINS
TRANSGLUTAMINASES
Resumé en anglais
The aims of the current study were: to standardize the detection of anti-tTg antibodies, comparing them with antiendomysial antibodies (EMA) and to assess the interaction of tTg with fibronectin. 49 celiac patients and 124 controls were enrolled. EMA was detected by indirect immunofluorescence reaction and tTg and fibronectin reactivity by in house ELISA and with commercially available kits. Seropositivity to anti-tTG was found in 46.9% and 100% of patients by the in house technique and by commercial kits, respectively. Fibronectin addition did not improve the ELISA sensibility. In conclusion, ELISA for anti-tTG detection has a high rate of false positive results and does not replace EMA
 
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Date de Publication
2005-10-05
 
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