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Doctoral Thesis
DOI
10.11606/T.5.2008.tde-10032008-111920
Document
Author
Full name
Luis Eduardo Becker
E-mail
Institute/School/College
Knowledge Area
Date of Defense
Published
São Paulo, 2008
Supervisor
Committee
Noronha, Irene de Lourdes (President)
Abensur, Hugo
Barretti, Pasqual
Jorgetti, Vanda
Pécoits Filho, Roberto Flávio Silva
Title in Portuguese
Efeito do paricalcitol e do calcitriol sobre a doença cardiovascular em camundongos uninefrectomizados ApoE -/-
Keywords in Portuguese
Aterosclerose
Calcitriol
Esclerose calcificante da média
Vitamina D/análogos & derivados
Abstract in Portuguese
O estudo investigou a influência do tratamento de 10 semanas com paricalcitol (0,1µg/kg 5x/semana) e calcitriol (0,03µg/kg 5x/semana) em modelo de aterosclerose espontânea utilizando camundongos ApoE -/- sham e uninefrectomizados (UNX). Resultados: a densidade capilar por comprimento no tecido cardíaco foi significativamente mais baixa nos animais UNX Controle quando comparados aos shams, o que não ocorreu nos animais UNX tratados com paricalcitol e calcitriol. Nas aortas, a relação parede/lúmen foi significativamente menor no grupo Sham Controle quando comparada à dos grupos UNX Controle e UNX Calcitriol, sendo que nesses últimos, foram evidenciadas calcificações vasculares acompanhadas por células positivas para Runx-2 (cbfa-1). Além disso, foi evidenciada uma menor expressão de TGFß nas aortas dos animais do grupo UNX Paricalcitol em relação aos grupos UNX Controle e UNX Calcitriol. Conclusões: Ambos os tratamentos preveniram alterações na capilarização cardíaca induzidas pela UNX. O tratamento com calcitriol na dose empregada induziu significativas calcificações vasculares, o que não ocorreu com o paricalcitol.
Title in English
Effect of paricalcitol and calcitriol on cardiovascular disease in uninephrectomized ApoE -/- mice
Keywords in English
Atherosclerosis
Calcitriol
Mönckeberg medial calcific
Vitamin D/analogues & derivatives
Abstract in English
The study investigated the influence of a 10-week treatment with Paricalcitol (0.1µg/kg, 5x/week) or Calcitriol (0.03µg/kg, 5x/week) on cardiovascular disease in spontaneously atherosclerotic ApoE -/- mice submitted to uninephrectomy (UNX). Results: capillary length density of the heart was significantly lower in UNX Control, but not in UNX Paricalcitol and UNX Calcitriol animals, when compared to shams. In the aortas, a significantly lower wall/lumen ratio was observed in the Sham Control group when compared to UNX Control and UNX Calcitriol groups. In the latter, vascular calcifications accompanied by a significant presence of Runx-2 (cbfa-1) positive cells was observed. TGFß aorta expression was significantly higher in UNX Control and UNX Calciriol groups when compared to UNX Paricalcitol. Conclusions: Both treatments were able to prevent the reduction in heart capillarization induced by the UNX model. Treatment with Calcitriol at the employed dose and duration, though, induced significant vascular calcifications.
 
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Publishing Date
2008-03-17
 
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