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Master's Dissertation
DOI
10.11606/D.5.2004.tde-19032007-120355
Document
Author
Full name
Márcia Regina Dutra do Valle
E-mail
Institute/School/College
Knowledge Area
Date of Defense
Published
São Paulo, 2004
Supervisor
Committee
Giron, Amilcar Martins (President)
Baracat, Fabio
Machado, Marcos Giannetti
Title in Portuguese
Análise morfométrica das fibras colágenas e reticulínicas na extrofia vesical
Keywords in Portuguese
Colágeno/análise
Coloração/métodos
Doenças da bexiga/cirurgia
Doenças da bexiga/patologia
Estudos de casos e controles
Estudos prospectivos
Interpretação estatística de dados
Reticulina/análise
Abstract in Portuguese
Trabalho prospectivo estudando a matriz extracelular da parede vesical em pacientes com extrofia vesical comparados ao grupo controle, pela microscopia óptica comum e luz polarizada com morfometria, quantificando-se as fibras colágenas e reticulínicas. Estudou-se 17 pacientes de ambos os sexos, biopsiando-se toda a parede vesical e empregando colorações HE, PS e Reticulina. Diferenças estatisticamente significantes foram notadas na análise quantitativa de fibras colágenas e reticulínicas o número de fibras colágenas foi significativamente maior e o número de fibras reticulínicas foi menor no grupo de pacientes com extrofia vesical quando comparado ao grupo controle.
Title in English
Morphometric analysis of collagen and reticulin fibers in classical bladder exstrophy
Keywords in English
Bladder diseases/pathology
Bladder diseases/surgery
Case-control studies
Collagen/analysis
Data Interpretation statistical
Prospective studies
Reticulin/analysis
Staining/methods
Abstract in English
A prospective study was done to evaluate the detrusor muscle's extracellular matrix in classical bladder exstrophy in comparison to a control group, by use of light microscopy and polarization method with morphometry, to quantify collagen and reticular fibers. Seventeen patients from both sexes were analysed and samples were obtained from the bladder and stained with Haematoxylin-eosin, Picrosirius red and the silver impregnation method. There were significant differences when comparing the quantity of collagen and reticular fibers. The collagen fibers were more abundant in the exstrophy bladders compared to controls, while the reticular fibers were present in smaller amounts.
 
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Publishing Date
2007-03-30
 
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