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Master's Dissertation
DOI
10.11606/D.58.2018.tde-23022018-135436
Document
Author
Full name
Arthur Cunha da Silva
E-mail
Institute/School/College
Knowledge Area
Date of Defense
Published
Ribeirão Preto, 2017
Supervisor
Committee
Nelson Filho, Paulo (President)
Martins, Lidia Parsekian
Matsumoto, Mirian Aiko Nakane
Ruellas, Antônio Carlos de Oliveira
Title in Portuguese
Avaliação in vivo dos efeitos genotóxicos/citotóxicos de disjuntores de Haas sobre células da mucosa bucal, pela análise de micronúcleos
Keywords in Portuguese
Citogenética; Expansão maxilar; Genotoxicidade; Testes para micronúcleos
Abstract in Portuguese
O objetivo do presente estudo foi avaliar os efeitos genotóxicos/citotóxicos ocasionados por disjuntores de Haas em células epiteliais esfoliadas da mucosa bucal de pacientes submetidos a tratamento ortodôntico, por meio do ensaio de micronúcleos. Participaram do estudo 22 pacientes entre 06 a 12 anos de idade, de ambos os gêneros, que necessitaram de disjuntores de Haas para correção de mordida cruzada posterior. Foi efetuada a coleta de células epiteliais da mucosa da bochecha, por meio de raspagem suave com escova científica. As células foram coletadas antes (T0), um mês após a instalação do aparelho (T1) e 3 meses após o travamento dos disjuntores (T2). As células foram processadas para obtenção de lâminas, as quais foram coradas com o método de Feulgen/Fast Green para quantificação do número de células normais, cariolíticas, picnóticas, brotos nucleares, bi/trinucleadas e com a presença de micronúcleos, em microscospia de luz. Os resultados foram submetidos à analise estatistica por meio do teste ANOVA, seguido do pós-teste de Tukey. O nível de significância adotado foi de 5%. Os resultados demonstraram que não houve diferença estatisticamente significante com relação à preença de micronúcleos, nos períodos avaliados (p>0,05). Os brotos nucleares sofreram aumento em T1 (p>0,05), com retorno aos níveis baseline em T2. Em relação às outras anormalidades (células cariolíticas, picnóticas e bi/trinucleadas), houve aumento significante em T1 e T2 (p<0,0001). Concluindo, este estudo demonstrou que os disjuntores de Haas não ocasionaram aumento de micronúcleos em células da mucosa bucal. Entretanto, foram observadas aumento estatisticamente significante das células cariolíticas, picnóticas e bi/trinucleadas durante o tratamento, sugerindo possíveis efeitos citotóxicos.
Title in English
Genotoxic/cytotoxic in vivo effects of Haas appliance by micronucleus assay in exfoliated mucosal cells
Keywords in English
Cytogenetic; Genotoxicity; Micronucleus tests; Palatal expansion technique
Abstract in English
The aim of the present study was to evaluate the genotoxic and cytotoxic effects caused by Haas appliance through micronuclei assay in buccal mucosa epithelial cells of patients submitted to orthodontic treatment. The study included 22 patients between 06 and 12 years of age, both genders, who required Haas appliance for correction of posterior crossbite. Epithelial cells from the cheek mucosa were collected performed by gentle scraping scientific toothbrush. The cells were collected before (T0), one month after the device was installed (T1) and 3 months after the appliance immobilization (T2). The cells were processed to obtain slides and Feulgen/Fast Green was used as staining method for counting the number of normal, karyolytic, pyknotic, nuclear buds, binucleated and micronucleus cells under light microscopy. The cellular abnormalities were evaluated with the parametric tests for comparison of the means by ANOVA test followed by the Tukey post-test. The significance level was 5%. The results of this study showed that there were no statistically significant results for the micronuclei in the evaluated periods (p>0,05). Nuclear buds increased in T1 (p<0,05), returning to baseline levels in T2. In relation to the other abnormalities (cariolytic, pyknotic and bi/trinucleated cells), there were a significant increase in T1 and T2 (p<0.0001). In conclusion, this study demonstrated that Haas appliance did not cause micronuclei increase in cells of buccal mucosa. However, a statistically significant increase in cariolytic, pyknotic and bi/trinucleated cells were observed during treatment, suggesting possible cytotoxic effects.
 
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Publishing Date
2018-09-13
 
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