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Master's Dissertation
DOI
https://doi.org/10.11606/D.6.2020.tde-12052020-140103
Document
Author
Full name
Valdson de Angelis Cortes
Institute/School/College
Knowledge Area
Date of Defense
Published
São Paulo, 1978
Supervisor
Committee
Paim, Gil Vianna (President)
Carvalho, João Pessoa de Paula
Qualifik, Paul
Title in Portuguese
Estudo da sensibilidade de um sistema biológico empregado no diagnóstico da raiva
Keywords in Portuguese
Raiva/Diagnóstico
Saúde Animal
Abstract in Portuguese
Quarenta e duas amostras de saliva de cães raivosos foram examinadas por inoculação intracerebral, para apreciar a sensibilidade do camundongo lactente em relação a do adulto jovem, no diagnóstico da raiva. O isolamento do vírus rábico de 100% das amostras utilizadas, nos dois sistemas biológicos, mostrou uma certa concordância dos resultados obtidos. A identificação de inclusões celulares e de antígeno rábico, mediante as técnicas de Sellers e de anticorpos fluorescentes, revelou uma perfeita regularidade desses indicadores da infecção rábica nas impressões encefálicas tanto de camundongos lactentes como de adultos jovens. O camundongo lactente revelou uma certa superioridade para revelar inclusões celulares e antígeno rábico, particularmente à imunofluorescência direta. A taxa de mortalidade mais elevada observada nos grupos de camundongos lactentes, revelou uma diferença altamente significante em relação ao mesmo evento verificado nos grupos de camundongos adultos jovens, evidenciando uma mais baixa resistência do camundongo lactente ao vírus rábico.
Title in English
Not available
Keywords in English
Not available
Abstract in English
42 saliva samples of rabid dogs were examined by intracerebral inoculation to apreciate the sensibility of suckling mouse in relation to the young adult in the diagnosis of rabies. Rabies vírus isolation of all of the samples utilized in the two biological sisterns showed certain agreement of results. Identification of cellular inclusions and rabies antigen by means of the technics of Sellers and fluorescent antibodies revealed a perfect regularity of these indicators of the rabies infection in the encephalic impressions of suckling mice and young adults. Suckling mouse were somewhat superior to reveal cellular inclusions and rabies antigen especially on direct inmunofluorescence. The higher death rate observed in the suckling mice groups was highly significant in relation to the same event verified in the young adult mice groups demonstrating a lower resistance of suckling mouse to rabies virus.
 
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MTR_233_Cortes_1978.pdf (867.52 Kbytes)
Publishing Date
2020-05-12
 
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