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Master's Dissertation
DOI
10.11606/D.60.2011.tde-28032012-153926
Document
Author
Full name
Carolina Bortolatti Vaccari
Institute/School/College
Knowledge Area
Date of Defense
Published
Ribeirão Preto, 2011
Supervisor
Committee
Tedesco, Antonio Claudio (President)
Ciancaglini, Pietro
Lopez, Renata Fonseca Vianna
Title in Portuguese
Estudos fotofísicos e fotobiológicos da ftalocianina NzPc e de nanotubos de carbono aplicáveis a processos fotodinâmicos
Keywords in Portuguese
Ftalocianinas
Nanotubos de Carbono
Terapia Fotodinâmica
Abstract in Portuguese
O câncer de pele, no Brasil, é responsavel por 25% de todos os tipos de câncer. Uma alternativa aos tratamentos usuais é a Terapia Fotodinâmica (TFD). Os processos fotodinâmicos em geral dependem da retenção de um composto fotossensível nos tecidos alvos e posterior irradiação com luz visível em comprimento de onda adequado. Após ativação, o fármaco tranfere energia para outras moléculas do meio, podendo gerar espécies como o oxigênio singlete (1O2) , radicais livres (O2,OH) e outras espécies reativas de oxigênio (EROs), essas, são responsáveis pelo processo fotooxidativo e indução da morte celular. Neste trabalho, o fármaco fotossensível NzPc (uma ftalocianina) foi estudado para verificar se suas características fotofísicas e fotoquímicas são adequadas a TFD, e, em seguida, foi associado a nanotubos de carbono de parede múltipla (NTCPM) funcionalizados com o tensoativo Pluronic F-127. Esse sistema de liberação de fármaco desenvolvido, foi caracterizado através de ensaios in vitro nas linhagens de melanoma de rato B16-F10 e carcinoma humano OSCC . O sistema NzPc/NTCPM/PF-127 apresentou atividade fotodinâmica superior ao fármaco na forma livre, possibilitando, maior interiorização do fármaco nas linhagens celulares e maior morte celular após irradiação.
Title in English
Studies of photophysical and photobiological of the NzPc phthalocyanine and carbon nanotubes used for photodynamic processes.
Keywords in English
Carbon nanotubes
Photodynamic Therapy
Phtalocyanines
Abstract in English
Skin cancer in Brazil are responsible for 25% of all cancers. An alternative to the usual treatments is photodynamic therapy (PDT). Photodynamic processes in general depend on the retention of a photosensitive compound in target tissues and subsequent irradiation with visible light at an appropriate wavelength. After activation, the drug, transfer energy to other molecules of the medium, which can generate species reactivate such as singlet oxygen (1O2), free radicals (O2 , OH) and other reactive oxygen species (ROS), these are responsible for photo-oxidative process and induction of cell death. In this work, NzPc photosensitive drug (a phthalocyanine) was studied to see if their photophysical and photochemical characteristics are suitable for PDT, and then was associated with multi-walled carbon nanotubes (MWCNT) functionalized with surfactant Pluronic F-127. This drug delivery system developed was characterized by in vitro assay on strains of mouse melanoma B16-F10 and carcinoma human OSCC. The system presented NzPc/NTCPM/PF-127 photodynamic activity than the drug in the free form, enabling greater internalization of the drug in cell and increased cell death after irradiation.
 
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Dissertacao.pdf (2.65 Mbytes)
Publishing Date
2013-05-08
 
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