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Master's Dissertation
DOI
10.11606/D.60.2008.tde-02102008-163106
Document
Author
Full name
Fernanda Peres Ferreira
Institute/School/College
Knowledge Area
Date of Defense
Published
Ribeirão Preto, 2008
Supervisor
Committee
Oliveira, Dioneia Camilo Rodrigues de (President)
Debonsi, Hosana Maria
Lopes, Marcia Nasser
Title in Portuguese
Análises químicas qualitativas e quantitativas de duas espécies de 'Mikania: Mikania glomerata' Sprengel e 'Mikania laevigata' Schultz Bip
Keywords in Portuguese
guaco.
Mikania glomerata
Mikania laevigata
Abstract in Portuguese
Mikania glomerata Sprengel e Mikania laevigata Schultz Bip, (Asteraceae, Eupatorieae, Mikaniinae), popularmente conhecidas como guaco, têm sido muito estudadas devido aos empregos medicinais das mesmas. São usadas para o tratamento da febre, reumatismo, gripe e doenças do trato respiratório. As monografias de ambas espécies encontram-se na Farmacopéia Brasileira. M. laevigata muitas vezes é confundida com M. glomerata, sendo comercializada como tal, ocorrendo principalmente no sul do país, onde este vegetal tem sua maior área de dispersão. Apesar de seu grande uso medicinal, sua fitoquímica clássica ainda tem se mostrado pouco estudada. Foi proposto neste trabalho o estudo fitoquímico das folhas secas de M. laevigata, sendo isoladas e identificadas por técnicas espectroscópicas as substâncias lupeol, taraxerol, cumarina, ácido orto-[(5-hidroxi)-cis-cinamoila]-transcinâmico, siringaldeído, trans-melilotosido, cis-melilotosido, 3,5-dicafeoilquinato de metila, 3,3,5 triidroxi 4,6,7- trimetoxiflavona, 3-O-b-D-glicosil-patuletina, 3-O-b-D-glicosilquercetina, 3-O-b-D-glicosil-caempferol, adenosina, ent-3a-O-b-D-glicopiranosídeo,16a,17- diidroxicaurano, e ácido 2b-[[3-O-(3-hidroxi-1-oxo-3-fenilpropil)-2-(3- metil-1- butiriloxi) - 4- O-(a-L-ramnopiranosil)-b- D-glicopiranosil] oxi]-13,15a-diidroxi-19-norcaur-16-en-18- óico. Além desses, identificou-se por GC-MS as substâncias ácido beierenóico, ácido caurenóico, óxido do cariofileno, espatulenol, diidrocumarina, e o acetato de lupeol. Foi proposta também a avaliação química dos extratos da lavagem foliar de M. glomerata e M. laevigata. A análise qualitativa desses extratos nos permitiu identificar como substâncias majoritárias, cumarina e lupeol, em M. laevigata, e lupeol e uma substância não identificada em M. glomerata. A padronização do método analítico em GC-MS permitiu a diferenciação das duas espécies, sendo utilizadas amostras de folhas de guaco de diversas regiões do país, e proposta suas identidades.
Title in English
Qualitative and quantivative chemical analysis of two species of Mikania: Mikania glomerata Sprengel and Mikania laevigata Schultz Bip.
Keywords in English
guaco.
Mikania glomerata
Mikania laevigata
Abstract in English
Mikania glomerata Sprengel and Mikania laevigata Schultz Bip, (Asteraceae, Eupatorieae, Mikaniinae), commonly known as guaco, have been studied because of their medicinal activities. They are used to treat fever, rheumatism, flu and respiratory tract diseases. The monographies of both species are found in Brazilian Pharmacopoeia. For many times, M. laevigata is confused with M. glomerata, and it has been sold as the last. This occurs mainly at the south of this country, where this plant has been found. In spite of the important medicinal uses from M. laevigata, the literature has few studies about its phytochemistry. In this work, the phytochemical study from the dried leaves of M. laevigata was proposed and the compounds lupeol, taraxerol, coumarin, orto-[(5-hydroxy)-cis-cinnamoyl]-transcinnamic acid, syringaldehyde, trans-melilotoside, cis-melilotoside, methyl 3,5-di-O-caffeoyl quinate, 3,3,5-trihydroxy-4,6,7-trimethoxyflavone, patuletin 3-O-b-D-glucopyranoside, quercetin 3-O-b-D-glucopyranoside, kaempferol 3-O-b-D-glucopyranoside, adenosine, entkaurane- 3a,16b,17 triol 3-O-b-D-glucopyranoside and 19-norkaur-16-ene-18-oic acid, 2b- [[3-O-(3-hydroxy -1-oxo -3 phenypropyl ) - 2 - ( 3- methyl - 1- oxobutyl - 4 - O - (a - Lramnopyranosyl) - b- D-glucopyranosyl]oxy]-13,15a-hidroxy were isolated and identified by spectroscopic methods. The compounds beyerenoic acid, kaurenoic acid, caryophyllene oxide, spathulenol dihydrocoumarin, and lupeol acetate were also identified, by GC-MS. It was proposed the chemical evaluation from the leaves rinse extracts of M glomerata and M. laevigata too. In the quantitative analysis of these extracts, coumarin and lupeol were identified as the main compounds of M. laevigata and lupeol and an unidentified compound of M. glomerata. The standardization of the analytical method by GC-MS allowed to differentiate one species from the other. Thus, leaves of guaco from many places of this country were used, and their identities were proposed.
 
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Publishing Date
2009-01-05
 
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