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Doctoral Thesis
DOI
10.11606/T.60.2008.tde-06062008-165805
Document
Author
Full name
Nilton Syogo Arakawa
Institute/School/College
Knowledge Area
Date of Defense
Published
Ribeirão Preto, 2008
Supervisor
Committee
Costa, Fernando Batista da (President)
Kato, Massuo Jorge
Pietro, Rosemeire Cristina Linhari Rodrigues
Rodrigues Filho, Edson
Said, Suraia
Title in Portuguese
Transformações microbianas e avaliação da citotoxicidade de lactonas sesquiterpênicas de 'Viguiera robusta' Gardn. (Asteraceae)
Keywords in Portuguese
Fungos de solo
Lactonas sesquiterpênicas
Transformações microbianas
Abstract in Portuguese
O processo de transformação microbiana de metabólitos secundários é uma técnica emergente no Brasil e que apresenta enorme potencial para obtenção de substâncias biotransformadas com novas e diferentes moléculas, as quais podem apresentar melhor desempenho de suas atividades biológicas. O objetivo do presente projeto foi efetuar transformações microbianas de metabólitos secundários oriundos da espécie vegetal Viguiera robusta Gardn. (família Asteraceae), com ênfase nas lactonas sesquiterpênicas (LSTs), para posterior avaliação de sua citotoxicidade frente a linhagens celulares. As etapas de isolamento e purificação de LSTs foram realizadas através de métodos cromatográficos e a elucidação estrutural através de métodos espectroscópicos. A avaliação da atividade citotóxica dos metabólitos biotransformados foi proposta em virtude de estudos iniciais indicarem potencial citotóxico da LST budleína A frente às linhagens SK-BR-3 (adenocarcinoma de mama) e células leucêmicas JURKAT.
Title in English
Microbial Transformations and cytotoxic evaluation of sesquiterpene lactones from Viguiera robusta Gardn. (Asteraceae).
Keywords in English
Asteraceae
Sesquiterpene lactones
Soil fungi
Abstract in English
The microbial transformation of secondary metabolites is an emerging technique in Brazil with high potential to obtain biotransformed compounds with new and different molecules which can show enhanced biological activities. Herein, microbial transformations of secondary metabolites from Viguiera robusta Gardn. (family Asteraceae), with emphasis in sesquiterpene lactones (STLs), were proposed with the aim to evaluate their cytotoxic activity against tumor cells lines. Isolation and purification of STLs were carried out through chromatographic methods and structural elucidation by spectroscopic methods. The evaluation of cytotoxic activity of the biotransformed metabolites was proposed due to the fact that previous studies indicated potential cytotoxic activity of the STLs budlein A against SK-BR-3 and JURKAT cells lines.
 
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Publishing Date
2008-10-29
 
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