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Master's Dissertation
DOI
10.11606/D.64.2009.tde-27042010-112316
Document
Author
Full name
Lucas William Mendes
E-mail
Institute/School/College
Knowledge Area
Date of Defense
Published
Piracicaba, 2009
Supervisor
Committee
Mui, Tsai Siu (President)
Andreote, Fernando Dini
Oliveira, Valéria Maia de
Title in Portuguese
Análise molecular das estruturas e diversidade de comunidades microbianas em solo de manguezal preservado da Ilha do Cardoso-SP
Keywords in Portuguese
ARISA
Ecologia microbiana
Manguezal
rRNA
T-RFLP
Abstract in Portuguese
Os manguezais tropicais são considerados um dos ecossistemas mais produtivos do mundo, sendo caracterizados pela alta taxa de ciclagem de matéria orgânica e nutrientes que ocorre entre os oceanos e os ambientes terrestres. Embora os manguezais sejam considerados áreas de proteção ambiental, a destruição desses ambientes é progressiva, devido a atividades industriais e portuárias nos estuários. Nos manguezais, a ciclagem de nutrientes está diretamente relacionada às atividades e a diversidade das comunidades microbianas presentes no solo. Este trabalho está inserido em um projeto mais amplo dentro do programa BIOTA/FAPESP, no que tange aos estudos da biodiversidade no Estado de São Paulo e utilização dessa biodiversidade de modo sustentável. O objetivo deste trabalho foi avaliar as estruturas e diversidade das comunidades de Bacteria, Archaea e Fungi presentes no solo de manguezal preservado da Ilha do Cardoso-SP. As amostras foram analisadas pelas técnicas de T-RFLP, ARISA, clonagem e seqüenciamento a fim de obter uma caracterização das estruturas das comunidades microbianas de uma área de manguezal preservado em comparação com os ambientes adjacentes de restinga e floresta e também a um manguezal antropizado. Os resultados permitiram concluir que o manguezal possui características exclusivas, com a presença de organismos distintos, revelando um possível potencial biotecnológico a ser explorado. Adicionalmente, os dados revelaram que a ação antrópica afetou as estruturas dessas comunidades de modo a ser notada uma sensível diminuição de diversidade no manguezal antropizado, evidenciando, dessa maneira, a importância da preservação desse ecossistema
Title in English
Molecular analysis of the structures and diversity of microbial communities in soil of preserved mangroves of Ilha do Cardoso-SP
Keywords in English
ARISA
Mangrove
Microbial ecology
rRNA
T-RFLP
Abstract in English
The tropical mangroves are considered one of the most productive ecosystems of the world, being characterized by the high tax of organic matter and recycling of nutrients, that happens between the oceans and the terrestrial habitats. Although the mangroves are considered areas of environmental protection, the destruction of those ecosystems is progressive, due to industrial and port activities in the estuaries. In mangroves, the recycling of nutrients is directly related to the activities and to the diversity of microbial communities present in the soil. This work is part of a wider project inside of the program BIOTA/FAPESP, with respect to the studies of the biodiversity in the State of São Paulo and use of that biodiversity in a maintainable way. The objective of this work was to evaluate the structures and diversity of communities of Bacteria, Archaea and Fungi present in the soil of preserved mangrove of Ilha do Cardoso-SP. The samples were analyzed by T-RFLP and ARISA techniques, cloning and sequencing in order to obtain a characterization of the microbial communities structure of preserved mangrove area in comparison with the adjacent environments of restinga (tropical moist forest) and forest and also to a degraded mangrove. The results allowed concluding that the mangroves present exclusive characteristics, with the presence of distinct organisms, revealing a possible biotechnological potential to be explored. Additionally, the data revealed that the human action affected the structures of those communities in a way to be noticed a sensitive diversity decrease in the degraded mangrove, evidencing, this way, the importance of the ecosystem preservation
 
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Mestrado.pdf (2.28 Mbytes)
Publishing Date
2010-04-27
 
WARNING: The material described below relates to works resulting from this thesis or dissertation. The contents of these works are the author's responsibility.
  • MENDES, Lucas William, et al. Shifts in phylogenetic diversity of archaeal communities in mangrove sediments at different sites an. Research in Microbiology [online], 2012. [acesso 2012-06-02]. Disponível em : <http://www.sciencedirect.com/science/article/pii/S0923250812000642>
  • NAVARRETE, Acácio Aparecido, et al. Molecular microbial ecology and conservation: A Molecular approach. In DE ARAúJO, Ademir Sérgio Ferreira, and FIGUEIREDO, Márcia do Vale Barreto. Microbial Ecology of Tropical Soils. 1 ed.. New York : Nova Science Publishers, Inc., 2011. chap. 5. p. 71-94.
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