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Doctoral Thesis
DOI
10.11606/T.75.2008.tde-05122008-171742
Document
Author
Full name
Lincoln Figueira Marins Coutinho
Institute/School/College
Knowledge Area
Date of Defense
Published
São Carlos, 2008
Supervisor
Committee
Lanças, Fernando Mauro (President)
Augusto, Fabio
Fernandes, João Batista
Reis, Boaventura Freire dos
Silva, José Carlos Rodrigues
Title in Portuguese
Desenvolvimento de instrumentação dedicada a cromatografia líquida capilar (cLC)
Keywords in Portuguese
cromatografia líquida capilar
injetor à base de tempo
instrumentação
programação de temperatura
Abstract in Portuguese
Desde que foi introduzida por Tswett no começo do século XX, a cromatografia vem sofrendo contínuos avanços. Entretanto, a miniaturização da cromatografia líquida, apesar de seu inicio promissor, ainda continua bastante lenta e até o presente não alcançou ampla difusão, sendo que o número de grupos trabalhando nesta área é ainda bastante restrito. O motivo deste lento avanço se encontrava na dificuldade em se desenvolver equipamentos adequados, colunas apropriadas e sistemas de tratamento de dados suficientemente rápidos para os sistemas miniaturizados. Apesar de muitos desses problemas atualmente serem de fácil resolução, ainda não se dispõe de equipamentos comercialmente disponíveis que supram satisfatoriamente às condições impostas pelas micro-colunas. Tal carência deve ser suprida antes de nos beneficiarmos de todas as vantagens intrínsecas à miniaturização da cromatografia líquida. Deste modo, o presente projeto visa o desenvolvimento de instrumentação totalmente dedicada aos sistemas miniaturizados de cromatografia líquida incluindo o desenvolvimento de uma bomba de alta pressão, um sistema de injeção a base de tempo, um forno capaz de realizar programação de temperatura e o software de controle dos mesmos. Os equipamentos desenvolvidos foram então aplicados em separações de estatinas demonstrando um excelente desempenho.
Title in English
Development of dedicated instrumentation to capillary liquid chromatography (cLC)
Keywords in English
capillary liquid chromatography
instrumentation
temperature programming
time-based injector
Abstract in English
Since the chromatography was introduced by Tswett, in the beginning of the 20th century, the technique has suffered a constant progress. However, the miniaturization of liquid chromatography, instead of its promising start, it is still too slow and this technique has not reached a wide divulgation so far. It is important to mention that the number of groups working in this area is very limited yet. The reason of this slow progress was due to the difficulty in developing suitable equipments, appropriated columns and data treatment systems that are quick enough for the miniaturized systems. Currently, many of these problems are easy to be solved, however, there are no equipments available commercially that supply the conditions imposed by microcolumns satisfactorily. Such lack should be filled before benefiting from all the advantages concerned to the miniaturization of liquid chromatography. In this way, this study aims at the development of instrumentation totally dedicated to the miniaturized systems of liquid chromatography, including the development of a high pressure pump, an time-based injector, an oven that sets the temperature programming and the software that can control all these devices. The developed equipments were then applied in the separation of statins, demonstrating an excellent performance.
 
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LincolCoutinho.pdf (21.96 Mbytes)
Publishing Date
2008-12-11
 
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