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Master's Dissertation
DOI
10.11606/D.75.2010.tde-12082010-081524
Document
Author
Full name
Janete Rodrigues Gonçalves
E-mail
Institute/School/College
Knowledge Area
Date of Defense
Published
São Carlos, 2010
Supervisor
Committee
Reis, Boaventura Freire dos (President)
Carrilho, Emanuel
Kamogawa, Marcos Yassuo
Title in Portuguese
Estudo da viabilidade da fotometria em fase sólida empregando multicomutação em fluxo, cela de fluxo com caminho óptico de 3mm e fotômetro de LED
Keywords in Portuguese
Cobalto
Fotometria em fase sólida
Multicomutação em fluxo
Abstract in Portuguese
No presente trabalho foi estudada a viabilidade da fotometria em fase sólida usando cela de fluxo com caminho óptico de 3 mm e fotômetro de LED, para determinação de cobalto em suplementos vitamínicos digeridos em meio ácido. O método automatizado foi baseado na formação de um composto de cobalto(II) com o reagente cromogênico 1-(2'-tiazolilazo)-2-naftol (TAN) retido em suporte sólido quimicamente modificado (C18) monitorado em 580 nm. O módulo de análise foi baseado no processo de multicomutação em fluxo. Após a definição das variáveis de controle, as amostras digeridas em meio ácido foram processadas. O procedimento permitiu a determinação de cobalto na faixa linear de 0,05 a 2,0 mg L-1. A precisão analítica, expressa como DPR, foi de 4,2% (n = 9) e freqüência de amostragem de 17 determinações por hora foram observadas. Os limites de detecção e quantificação foram estimados em 0,0166 e 0,055 mg L-1, respectivamente.
Title in English
Study of the feasibility of using solid-phase photometry employing multicommutation, flow cell with 3mm optical path and LED photometer
Keywords in English
Cobalt
Multicomutation
Solid-phase spectrophotometry
Abstract in English
In the present work the viability of the solid phase photometry using a flow cell with optical pathway of 3 mm and a LED photometer for the determination o cobalt vitamin supplements digested in acidic medium was studied. The method automated was based on the compound formation of cobalt and the chromomeric reagent 1-(2'-tiazolilazo)-2-naftol (TAN), which was immobilized on a solid support (C18) and monitored at 580 nm. The analysis module was based on the multicommutation flow analysis process. After definition of the variables control, samples digested in acid medium ware analyzed. The procedure allowed cobalt determination within the linear range 0.0 up to 2.0 mg L-1. The analytical precision, expressed as DPR, was 4.2 %, and the analytical frequency was 17 determinations per hour. Limit of detection and limit of determination were 0.0166 and 0.055 mg L-1, respectively.
 
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Publishing Date
2010-12-01
 
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