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Master's Dissertation
DOI
10.11606/D.76.1997.tde-29012010-154314
Document
Author
Full name
Maria do Rosário Zucchi
Institute/School/College
Knowledge Area
Date of Defense
Published
São Carlos, 1997
Supervisor
Committee
Bonagamba, Tito Jose (President)
Colnago, Luiz Alberto
Magon, Claudio Jose
Title in Portuguese
Implementação da técnica de espectroscopia in vivo por RMN e sua aplicação na fisiologia do exercício.
Keywords in Portuguese
Espectroscopia in vivo
Exercício
RMN
Abstract in Portuguese
A Espectroscopia por Ressonância Magnética Nuclear (RMN) tem oferecido inúmeras possibilidades de pesquisa nas áreas de Biologia e Medicina. Uma de sus principais aplicações é a Espectroscopia in vivo do 31P no estudo da fisiologia de músculos esqueléticos. A partir desta metodologia pode-se quantificar as concentrações dos diferentes metabólitos fosforados que participam do metabolismo energético dos músculos, bem como suas alterações em função de contrações musculares. Com o objetivo de implantar a técnica de Espectroscopia in vivo no Laboratório de RMN do IFSC/USP, introduzimos várias adaptações ao Espectrômetro de Alta Resolução em Sólidos já existentes, incluindo a confecção de sondas . A partir desta adaptação, estudamos as alterações metabólicas dos compostos fosforados presentes em músculos esqueléticos, bem como o pH intraceleular, de ratos e camundongos em função de estímulos elétricos e exercício físico intenso.
Title in English
Developing of in vivo NMR spectroscopy technique and its applications in exercise physiology.
Keywords in English
Exercise
In vivo spectroscopy
NMR
Abstract in English
The Nuclear Magnetic Resonance (NMR) spectroscopy has been offering many possibilities of research in the areas of Biology and Medicine. One of the most important applications is the 31P in vivo Spectroscopy to study the skeletal muscle physiology. Using this methodology, it is possible to quantify different phosphorus metabolite concentrations that take part of the muscle energetic metabolism, as their variations as a function of muscle contraction. To accomplish the objective of developing the in vivo spectroscopy technique in the NMR laboratory of the IFSC/USP, many modifications to our Solid High Resolution Spectrometer were introduced and probes were constructed. Following these changes, we studied phosphorus compounds metabolic changes in the skeletal muscle and the intracellular pH of rats and mice as a function of electric stimulation and intensive running exercise.
 
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Publishing Date
2010-03-08
 
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