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Master's Dissertation
DOI
10.11606/D.82.2001.tde-09102001-162859
Document
Author
Full name
Dyjalma Antonio Bassoli
Institute/School/College
Knowledge Area
Date of Defense
Published
São Carlos, 2001
Supervisor
Committee
Ferreira, Affonso Luiz (President)
Almeida, Simone Acrani de
Duarte, Luiz Romariz
Title in Portuguese
Avaliação dos efeitos do ultra-som pulsado de baixa intensidade na regeneração de músculos esqueléticos com vistas à aplicabilidade em clínica fisioterapêutica.
Keywords in Portuguese
regeneração muscular
ultra-som pulsado de baixa intensidade
Abstract in Portuguese
Foi utilizado na presente pesquisa o ultra-som pulsado de baixa intensidade, com a finalidade de avaliar, experimentalmente, seus efeitos físicos sobre a regeneração do músculo esquelético de ratos. Foram empregados no experimento 10 animais de ambos os sexos. Após eleição do músculo glúteo maior, foram feitas incisões perpendiculares (pós anestesia) às suas fibras, alcançando aproximadamente dois terços de sua espessura. Seguinte às incisões, realizou-se síntese do músculo e dos planos superficiais. Foi convencionado estudar os efeitos do ultra-som pulsado após 3, 6 e 10 dias de estímulo. Antes do sacrifício dos animais foi-lhes administrado Colchicina, com a finalidade de bloquear a células em divisão. Mediante análise histopatológica comparativa dos músculos, constatou-se que enquanto nos animais não estimulados desenvolveu-se, aos 3 dias, áreas de necrose e inflamação, naqueles estimulados houve uma diminuição das mesmas. Além disso, nos animais estimulados, ocorre intensa neovascularização nas áreas de lesão. Observou-se também uma migração de células miogênicas (satélites) para restos de bainhas remanescentes de fibras musculares regeneradas. Constatou-se um aumento de mioblastos com núcleos em metáfase. Aos 10 dias, os animais estimulados exibiam inúmeros miotubos com formação de miofibrilas, caracterizando células musculares em franca maturação. Longe da área estimulada, o ultra-som foi capaz de induzir mitoses em células satélites. Notou-se que os efeitos do ultra-som pulsado aceleram a regeneração de fibras musculares mediante uma intensa neoformação vascular, acelerando a reparação da necrose e formação de mioblastos, os quais agregam-se em miotubos formando novas células.
Title in English
Evaluation of the effects of low intensity pulsed ultrasound on skeletal muscle regeneration having in mind its applicability to physiotherapeutic clinic.
Keywords in English
low intensity pulsed ultrasound
muscle regeneration
Abstract in English
It was used in the present research the low intensity pulsed ultrasound with aim of evaluating, experimentally, its physical effects on the regeneration of rats skeletal muscle. Ten male and female animals were used in this experiment. After election of the gluteus maximus muscle, perpendicular incisions were made (after anesthesia) in its fibers, reaching approximately two thirds of its thickness. After the incisions, a synthesis of the muscles and superficial plans was performed. It was established to study the effects of the pulsed ultrasound after 3, 6 and 10 day-stimuli. Before sacrificing the animals Colchinine was given aiming to block cells in division. By means of comparative histopathological analysis of the muscles, it was verified that in non-stimulated animals an area of necrosis developed in three days, while in those that were simulated there was a decrease of the same. Moreover, on the stimulated animals, there is intense neovascularization in the damaged area. A migration of myogenic cells (satellites) to rests of remaining sheaths from regenerated muscle fiber was also observed. It was verified an increase of myoblasts with nuclei in metaphase. In ten days, the stimulated animals showed countless myotubes with formations of myofibrils, characterizing mature muscle cells. Far from the stimulated area, the ultrasound was able of inducing mitosis to satellite cells. It was noticed that the effects of the pulsed ultrasound accelerate muscle fiber regeneration by means of an intense vascular neoformation, accelerating the restoration of the necrosis and the formation of myoblasts, which aggregates in myotubes forming new cells.
 
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Publishing Date
2001-11-07
 
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