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Master's Dissertation
DOI
10.11606/D.82.2004.tde-19012005-103055
Document
Author
Full name
Rosemary Aparecida de Oliveira
E-mail
Institute/School/College
Knowledge Area
Date of Defense
Published
São Carlos, 2004
Supervisor
Committee
Plepis, Ana Maria de Guzzi (President)
Assis, Odilio Benedito Garrido de
Martins, Virginia da Conceicao Amaro
Title in Portuguese
Avaliação do efeito antimicrobiano in vitro de quitosana e da associação quitosana/clorexidina sobre saliva e Streptococcus mutans
Keywords in Portuguese
atividade antimicrobiana
clorexidina
quitosana
saliva
Streptococcus mutans
Abstract in Portuguese
Saliva e Streptococcus mutans foram utilizados neste estudo para avaliar a atividade antimicrobiana da quitosana associada à clorexidina. Os testes de atividade antimicrobiana foram realizados utilizando-se soluções de quitosana e quitosana:clorexidina preparadas em ácido acético 1%, em diversos valores de pH (3,5-5,0) e valores de concentração variando entre 0,1 e 1,5% (m/v). Antes de cada experimento o pH da solução estoque de quitosana (grau de desacetilação 76% e massa molar 400.000 g/mol) foi ajustado até o pH desejado pela adição de NaOH. A atividade antimicrobiana aumentou com o pH, com a concentração, com o tempo de exposição e com a associação quitosana/ clorexidina. Aumentando-se a concentração de clorexidina não se observam efeitos significativos, indicando que em presença de quitosana é possível liberar este composto em concentrações menores, o que irá minimizar os efeitos colaterais indesejados de pigmentação e sensação de alteração de sabor
Title in English
Evaluation of in vitro antimicrobial effect of chitosan and chitosan/chlorhexidine on saliva and Streptococcus mutans
Keywords in English
antimicrobial activity
chitosan
chlorhexidine
saliva
Streptococcus mutans
Abstract in English
Saliva and Streptococcus mutans were used to evaluate antimicrobial activity of chitosan/chlorexidine solutions. Antimicrobial tests were conducted using chitosan and chitosan/ chlorexidine solutions in 1.0 % acetic acid solution, at various pH values (3.5-5.0) and concentrations ranging from 0.1 to 1.5% (w/v). Prior to each experiment, chitosan (DA 76%, 400.000 g/mol) stock solution pH was adjusted to the desired pH using NaOH. The antimicrobial activity increased with pH, concentration, exposition time and with the association of chitosan and chlorexidine. Increasing the concentration of chlorexidine did not have a significant effect indicating that in presence of chitosan it is possible to deliver this compound at a lower concentration which will avoid unwanted side effects including staining and altered taste sensations
 
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Publishing Date
2005-06-30
 
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