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Master's Dissertation
DOI
10.11606/D.87.2015.tde-01092015-203528
Document
Author
Full name
Daniella Aparecida Franze
Institute/School/College
Knowledge Area
Date of Defense
Published
São Paulo, 2014
Supervisor
Committee
Battesti, Darci Moraes Barros (President)
Moraes Filho, Jonas
Piazza, Roxane Maria Fontes
Title in Portuguese
Cultura de células embrionárias-simile de Rhipicephalus sanguineus (Latreille) (Acari: Ixodidae) para isolamento e cultivo de patógenos.
Keywords in Portuguese
Rhipicephalus sanguineus
Células embrionárias-simile
Cultivos primários
Linhagem celular
Patógenos
Abstract in Portuguese
Diversas linhagens de células de carrapatos já foram estabelecidas em outras regiões do mundo para isolamento de patógenos. O objetivo deste estudo é obter cultivos de células de Rhipicephalus sanguineus para testar o crescimento de alguns bioagentes. O primeiro capítulo trata do estabelecimento da linhagem celular e o segundo, do uso da linhagem como substrato para infecção de patógenos. Ovos de R. sanguineus foram preparados em meio L-15B e mantidos à 30 °C. Quando as células se tornaram confluentes, as culturas foram propagadas e criopreservadas. O descongelamento foi bem sucedido a partir da terceira passagem e a identidade celular foi confirmada por sequenciamento utilizando o fragmento 16S rDNA mitocondrial. As células foram infectadas com Erhlichia canis, Leishmania infantum chagasi e Trypanosoma cruzi, sendo eficientes somente para E. canis.
Title in English
Tick embryonic-like cell culture of Rhipicephalus sanguineus (Latreille) (Acari: Ixodidae) for pathogen isolation and cultivation.
Keywords in English
Rhipicephalus sanguineus
Cell line
Embryonic-like cell
Pathogens
Primary cultures
Abstract in English
Several lines of embryonic cells of ticks already been established in other regions of the world, and are used to isolate and propagate pathogens. The aim of this study, is to obtain cell cultures from Rhipicephalus sanguineus to test the growth of some bioagents.The first addressing consists of the establishment of the cells and the second, using the cell lineage as a substrate for the pathogens infection. The egg masses of R. sanguineus were prepared in L-15B medium and cultures were maintained at 30 °C. When a confluent cellular monolayer was obtained, the cultures were passaged and frozen. Defrosting of cryopreserved cultures from the third passage was successful. Cell identity was confirmed by sequencing using 16S rDNA gene fragment. Cells were infected with Erhlichia canis, Leishmania infantum chagasi and Trypanosoma cruzi. Only for E. canis the cells of R. sanguineus were effective as a substrate for growth of this pathogen.
 
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Release Date
2017-08-31
Publishing Date
2015-09-04
 
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