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Master's Dissertation
DOI
10.11606/D.87.2010.tde-03032011-104355
Document
Author
Full name
Simone Cristina Yamasaki
Institute/School/College
Knowledge Area
Date of Defense
Published
São Paulo, 2010
Supervisor
Committee
Silveira, Paulo Flavio (President)
Azevedo, Anita Mitico Tanaka
Fernandes, Cristina Maria
Title in Portuguese
Efeitos do veneno de Apis mellifera e do metotrexato sobre peptidases do plasma, do líquido e membrana sinoviais e de leucócitos circulantes em ratos com artrite induzida por colágeno tipo II.
Keywords in Portuguese
Artrite animal
Ativação enzimática
Colágeno
Peptídeos
Ratos
Venenos de origem animal
Abstract in Portuguese
Atividades peptidásicas têm sido relacionadas à artrite reumatoide (AR). Os mecanismos de ação do tratamento usual, o metotrexato (MTX), e de terapias alternativos da AR, como a acupuntura com veneno de abelhas (BV), não são completamente compreendidos. Para contribuir com o conhecimento da AR, o presente estudo avaliou parâmetros consagrados para AR e atividades de aminopeptidases básica (APB), neutra (APN) e dipeptidil peptidase 4 (DPP4), e da endopeptidase prolil oligopeptidase (POP) em amostras de animais controle e submetidos à indução de artrite por colágeno (CIA) com ou sem tratamento sistêmico com BV e/ou MTX, e em animais resistentes, bem como efeitos diretos do BV ou do MTX sobre as atividades de aminopeptidases sob estudo. Em conclusão: atividades aminopeptidásicas estão envolvidas na CIA e podem ser potencialmente úteis na avaliação diagnóstica e prognóstica da AR; os tratamentos com BV e/ou MTX são capazes de amenizar ou normalizar essas alterações nas aminopeptidases; o BV não tem efeito analgésico, mas é capaz de diminuir o TNF-a; o BV pode conter algum inibidor da APN, enquanto o MTX parece agir como um inibidor não seletivo de APB, APN e DPP4.
Title in English
Effects of Apis mellifera venom and methotrexate on plasma, synovial fluid and membrane and circulating leukocytes peptidases in rats with type II collagen- induced arthritis.
Keywords in English
Animal arthritis
Animal poisons
Collagen
Enzyme activation
Mice
Peptides
Abstract in English
Peptidase activities have been related to rheumatoid arthritis (RA). The mechanisms of action of the usual treatment, methotrexate (MTX), and alternative therapies for RA, such as bee venom (BV) acupuncture, are not completely known. To contribute to the understanding of RA, this study evaluated parameters of RA and activities of basic (APB), neutral (APN) and dipeptidyl peptidase 4 (DPP4) aminopeptidases, and oligopeptidase prolyl endopeptidase (POP) in samples from rats with collagen-induced arthritis (CIA) and in control rats with or without systemic treatment with BV and /or MTX and in resistant animals, as well as the direct effects of BV or MTX on the aminopeptidase activities under study. In conclusion: aminopeptidase activities are involved in CIA and may be potentially useful in the diagnostic and prognostic evaluation of RA; treatment with BV and / or MTX are able to reduce or normalize these aminopeptidase alterations; BV has no analgesic effect, but it is able to decrease TNF-a; BV may contain some inhibitor of APN, while the MTX seems to act as a nonselective inhibitor of APB, APN and DPP4.
 
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Publishing Date
2011-08-11
 
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