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Doctoral Thesis
DOI
10.11606/T.87.2018.tde-08022018-115424
Document
Author
Full name
Elisa Chaparro Aguirre
Institute/School/College
Knowledge Area
Date of Defense
Published
São Paulo, 2017
Supervisor
Committee
Silva Junior, Pedro Ismael da (President)
Fukushima, Caroline Sayuri
Guimaraes, Margareth de Lara Capurro
Miranda, Antonio de
Nakano, Eliana
Title in Portuguese
Estudo da resposta imune inata em quilópodes (Scolopendromorpha Myriapoda).
Keywords in Portuguese
Centípede
Peptídeo antimicrobiano
Sistema imune
Transcriptoma
Abstract in Portuguese
Os peptídeos bioactivas são fundamentais no sistema imune inato (SII), devido a que eles proveem informação importante sobre o SII. O objetivo deste estudo é caracterizar os componentes e reações do SII em quilópodes. Para isto, analisamos o transcriptoma dos hemócitos de Scolopendra subspinipes subspinipes; assim como também, extraímos, purificamos e caracterizamos os peptídeos antimicrobianos presentes na exúvia, o extrato total e a hemolinfa de S. s. subspinipes e S. viridicornis. Várias frações com atividade antimicrobiana foram isoladas da hemolinfa. Dois novos peptídeos antimicrobianos foram caracterizados e sintetizados: a Pinipesina, de S. s. subspinipes e a Lacraina, de S. viridicornis. A análise preliminar do transcriptoma apresentou que os contigs relacionados ao SII foram transcritos com mais frequência moléculas com atividade regulatória (33,7%) e de reconhecimento (11,6%), e as relacionadas a cascata de coagulação (21,4%). Uma análise mais completa do transcriptoma e das moléculas bioactivas é apresentado.
Title in English
Study of the innate immune response in Chilopoda (Scolopendoromorpha, Myriapoda).
Keywords in English
Antimicrobial peptides
Centipede
Immune system
Transcriptome
Abstract in English
Bioactive peptides are vital in the innate immune system (IIS), because they provide substantial information about the IIS. This study aims to characterize the components and reactions that constitute the ISS in Chilopoda. For this, we analyzed the hemocytes transcriptome from Scolopendra subspinipes subspinipes; as also extracted, purified and characterized the bioactive peptides presents in the exuviae, the body extract and the hemolymph from S. s. subspinipes and S. viridicornis. Several fractions with antimicrobial activity from the hemolymph were isolated. Two new antimicrobial peptides were characterized and synthetized as well: The Pinipesin, from S. s. subspinipes, and the Lacrain, from S. viridicornis. The preliminary analysis from the transcriptome show that the contigs related to the IIS were transcribed more frequently molecules with regulatory (33,7 %) and recognition (11,6%) activities, and those involved in the coagulation cascade (21,4%). A more complete analysis of the transcriptome and the bioactive molecules is presented.
 
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Release Date
2020-02-08
Publishing Date
2018-02-08
 
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