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Master's Dissertation
DOI
10.11606/D.87.2013.tde-17052014-084116
Document
Author
Full name
Gabrielle Ribeiro de Andrade
Institute/School/College
Knowledge Area
Date of Defense
Published
São Paulo, 2013
Supervisor
Committee
Domingos, Marta de Oliveira (President)
Barazzone, Giovana Cappio
Vaz, Tania Mara Ibelli
Title in Portuguese
Resposta imune induzida por uma vacina conjugada contra Escherichia coli diarreiogênicas pertencentes ao sorogrupo O111.
Keywords in Portuguese
Escherichia coli
Diarréia
Polissacarídeos
Vacinas
Virulência
Abstract in Portuguese
E. coli pertencentes ao sorogrupo O111 são incluem amostras com diferentes fatores de virulência que são responsáveis por gastroenterites por todo o mundo e surtos de diarreia com sangue e síndrome hemolítico-urêmica em países desenvolvidos. Resultados obtidos anteriormente mostraram que o LPS O111 é um bom candidato a antígeno para a formulação de uma vacina contra E. coli O111. Desta forma, o polissacarídeo O111 foi conjugado com proteínas arreadoras. Coelhos foram imunizados pela via subcutânea com o conjugado O111-citocromo c incorporado em sílica SBA-15 como adjuvante, os resultados mostraram que os anticorpos obtidos foram capazes de reconhecer e inibir a adesão de todas as categorias de E. coli pertencentes ao sorogrupo O111. O veículo carreador de antígeno, VaxcineTM , foi incorporado no conjugado O111-EtxB e camundongos foram imunizados pela via oral, o que resultou em resposta imune sistêmica e de mucosa contra o LPS O111. Os anticorpos obtidos foram capazes de reconhecer amostras de todas as categorias de E. coli O111. Os dados obtidos neste trabalho sugerem que a utilização do polissacarídeo O111 em uma formulação conjugada é capaz de prevenir surtos de diarreia causada por E. coli O111 independente do seu mecanismo de virulência.
Title in English
Immune responses induced by a conjugate vaccine against diarrheagenic Escherichia coli belonging to serogroup O111.
Keywords in English
Escherichia coli
Diarrhea
Polysaccharides
Vaccines
Virulence
Abstract in English
The E. coli serogroup O111 include samples with different virulence factors that are responsible for enteritis throughout the world and for outbreaks of bloody diarrhea and hemolytic uremic syndrome in developed countries. Previous results obtained have shown that O111 LPS is a good candidate antigen for the development of a vaccine against E. coli O111. Therefore, the polysaccharide was conjugated with carrier proteins. Rabbits were immunized subcutaneously with the O111-cytochrome c conjugate incorporated in silica SBA-15 as an adjuvant, the results obtained show that the antibodies were able to recognize and inhibit the adhesion of all types of E. coli belonging to serogroup O111. The antigen delivery vehicle, VaxcineTM was incorporated into the O111-EtxB conjugate, and mice were immunized by gavage, resulting in systemic and mucosal immune response against O111 LPS. These antibodies were able to recognize all categories of E. coli O111. The results presented in this work indicate that an oral conjugated vaccine that targets the O111 antigen has the potencial to prevent diarrhea by O111 E. coli strains, regardless their mechanism of virulence.
 
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Publishing Date
2014-05-21
 
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