Produção de anticorpos monoclonais para caracterização de variantes antigênicas brasileiras de vírus da raiva.

Chaves, Luciana Botelho

doi:10.11606/T.87.2010.tde-19082010-165840

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Doctoral Thesis

DOI

https://doi.org/10.11606/T.87.2010.tde-19082010-165840

Document

Doctoral Thesis

Author

Chaves, Luciana Botelho (Catálogo USP)

Full name

Luciana Botelho Chaves

Institute/School/College

Interunidades em Biotecnologia

Knowledge Area

Biotechnology

Date of Defense

2010-05-10

Published

São Paulo, 2010

Supervisor

Gaspari, Elizabeth Natal de (Catálogo USP)

Committee

Gaspari, Elizabeth Natal de (President)
Brandão, Paulo Eduardo
Lazarini, Silvana Regina Favoretto
Pereira, Carlos Augusto
Zanetti, Carlos Roberto

Title in Portuguese

Produção de anticorpos monoclonais para caracterização de variantes antigênicas brasileiras de vírus da raiva.

Keywords in Portuguese

Anticorpos monoclonais
Caracterização antigênica
Morcegos insetívoros
Vírus da raiva

Abstract in Portuguese

Anticorpos monoclonais (AcMo) contra proteínas do vírus da raiva (RABV) foram produzidos para adequar a caracterização antigênica dos isolados no Brasil. Foram selecionados dois isolados de morcegos insetívoros, sendo um de Nyctinomops laticaudatus e outro de Eptesicus furinalis que apresentaram perfis não compatíveis (NC) com os pré-estabelecidos. As suspensões virais foram adaptadas para crescimento em cultura de células N2A. Para o preparo de AcMo foram utilizadas como antígeno as ribonucleoproteínas dos isolados selecionados. Foram obtidos dois AcMo, o 3A7 e o 4E10. Analisando 57 isolados de RABV com esses AcMo, o 3A7 reagiu com 21 (36,84%) e o 4E10 com 25 (43,85%). Dos 13 isolados caracterizados como variante antigênica 3 (Desmodus rotundus) o 3A7 reagiu com 8 (61,53%) e o 4E10 com 11 (84,61%). Dos 9 isolados com perfil NC em morcegos o 3A7 reagiu com 5 (55,55%) e o 4E10 com 4 (44,44%). Os anticorpos produzidos poderão auxiliar na complementação do painel existente de caracterização antigênica o que poderá aprimorar a vigilância epidemiológica da doença.

Title in English

Production of monoclonal antibodies for characterization of brazilian antigenic variants of rabies virus.

Keywords in English

Antigenic characterization
Insectivorous bats
Monoclonal antibodies
Rabies virus

Abstract in English

Monoclonal antibodies (MAb) against the rabies virus (RABV) proteins were produced to improve the antigenic characterization of the isolates in Brazil. Two isolates from insectivorous bats were selected; one was from the species Nyctinomops laticaudatus and the other from Eptesicus furinalis, which showed non-compatible (NC) profiles from pre-established ones. The viral suspensions were adapted for growth in N2A cells. Ribonucleoproteins from selected isolates were used as antigen for the preparation of Mab. We obtained two Mab, the 3A7 and the 4E10. Of the 57 RABV isolates analyzed with these MAb, the 3A7 reacted with 21 (36.84%) and 4E10 with 25 (43.85%). Of the 13 isolates characterized as antigenic variant 3 (Desmodus rotundus), the 3A7 MAb reacted with 8 (61.53%) and 4E10 with 11 (84.61%). Of the nine isolates with the profile NC of bats the 3A7 reacted with 5 (55.55%) and the 4E10 with 4 (44.44%). The antibodies produced may help to complement the existing panel to antigenic characterization which could improve the disease epidemiological surveillance.

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Publishing Date

2010-09-28

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