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Master's Dissertation
DOI
10.11606/D.87.2016.tde-20102016-094519
Document
Author
Full name
Renan Lima de Araujo
Institute/School/College
Knowledge Area
Date of Defense
Published
São Paulo, 2016
Supervisor
Committee
Mendonça, Ronaldo Zucatelli (President)
Ishida, Kelly
Silva Junior, Pedro Ismael da
Title in Portuguese
Estudo do potencial antimicrobiano do muco de Phyllocaulis boraceiensis.
Keywords in Portuguese
Bioprospecção
Lesma
Muco
Peptídeos antimicrobianos
Sequenciamento \"de novo\"
Abstract in Portuguese
Os peptídeos antimicrobianos tem sido alvo de cada vez mais estudos como candidatos a antibióticos naturais devido a seu amplo espectro de ação e baixa suceptibilidade a induzir resistência microbiana. Carentes de sistema imune específico, os invertebrados contam com um eficiente sistema imune inato para se defenderem de microrganismos patogênicos, incluindo uma coleção de peptídeos antimicrobianos. Através de cromatografia, ensaios antimicrobianos, espectrometria de massas e análise em banco de dados, isolou-se frações do muco de lesmas da espécie P boraceiensis com efeito antimicrobiano e obteve-se sequencias mais prováveis de fragmentos de algumas das frações ativas encontradas, relacionando-os as proteínas e peptídeos conhecidos mais similares e sugerindo relação entre as sequencias encontradas, as proteínas e peptídeos similares, bem como possível relação com atividade antimicrobiana. Obteve-se uma gama de frações ativas contra bactérias e/ou leveduras e sequências e informações relacionadas que podem ser úteis para futuros estudos de isolamento e caracterização de fatores antimicrobianos no muco de P boraceiensis.
Title in English
Antimicrobial potential of Phylocaullis boraceiensis mucus.
Keywords in English
De novo sequencing
Antimicrobial peptides
Bioprospection
Mucucs
Slug
Abstract in English
Antimicrobial peptides are becoming increasingly more important as natural antibiotics candidates since they show a broad spectrum of action and low suceptibility to induce microbial resistance. Lacking specific imunne system, invertebrates count with an efficient innate imunne system to deffend theirselves from pathogenic microrganisms, including a collection of antimicrobial peptides. Through chromatografy, antimicrobial tests, mass spectometry and database analysis, we isolated fractions from the mucucs of P boraceiensis slugs showing antimicrobial effect. We obtained most propable sequences from several active fractions, relating them with most similar known peptides and proteins. There were sugesteg some possible connections between the found sequences and related peptides and proteins as well as possible antimicrobial activity relations. We found several active fractions against bacteria and/or yeast as well as sequences and related information that may be usefull in further isolation and caracterization studies of antimicrobial factors in P boraceiensis mucus.
 
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Publishing Date
2016-10-20
 
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