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Master's Dissertation
DOI
https://doi.org/10.11606/D.87.2018.tde-23022018-164103
Document
Author
Full name
Ana Beatriz Contarelli Mezei
Institute/School/College
Knowledge Area
Date of Defense
Published
São Paulo, 2017
Supervisor
Committee
Franzolin, Marcia Regina (President)
Franco, Milene Tino de
Neves, Carla Taddei de Castro
Title in Portuguese
Perfil de resistência a antimicrobianos de amostras de Escherichia coli enteropatogênica atípica.
Keywords in Portuguese
β-lactamases de espectro estendido (ESBL)
Escherichia coli
Diarreia
EPEC atípica
Resistência antimicrobiana
Abstract in Portuguese
A Escherichia coli enteropatogênica atípica (aEPEC) é um dos principais agentes causadores de diarreia infantil e às vezes é necessário o tratamento com antimicrobianos. O objetivo do presente trabalho foi verificar o perfil de resistência aos antimicrobianos de 72 amostras de aEPEC, correlacionando com o perfil genético de resistência e a produção de β-lactamases de espectro extendido (ESBL). As aEPEC apresentaram resistência aos β-lactamâmicos, sulfonamidas, tetraciclinas, aminoglicosídeos e ansamicina, verificadas através de disco difusão. Multirresistência foi identificada em 27,8%. Treze amostras (18,1%) produziram ESBL. Os genes de resistência encontrados através de PCR foram: sul1 - 50%, sul2 - 86,4%, tetA - 42,1%, tetB - 68,4%, tetC- 5,3%, blaCTX-M - 26,1%, blaTEM -78,3%, blaSHV - 4,4% e intl - 35,7%. O conhecimento sobre o perfil de resistência e produção de ESBLs é muito importante na orientação do tratamento adequado quando se fizer necessário, além de conhecer o potencial de resistência que poderiam vir a ser transferidos para outras bactérias.
Title in English
Antimicrobial resistance profile of atypical Enteropathogenic Escherichia coli strains.
Keywords in English
Escherichia coli
Antimicrobial resistance
Atypical EPEC
Diarrhea
Extended spectrum-β-lactamases (ESBL).
Abstract in English
The atypical Enteropathogenic Escherichia coli (aEPEC) is one of the most common childhood diarrhea`s agent, and sometimes the antimicrobials treatment may be necessary. The aim of this study was verify the antimicrobial resistance profile of 72 aEPEC strains, correlating with the resistance genetic profile, and the extended-spectrum β-lactamases (ESBL) production. The aEPEC strains presented resistance to β-lactams, Sulfonamides, Tetracycline, Aminoglycoside, and Ansamycin, verified through disc diffusion. Multiresistance was identified in 27.8%. Thirteen strains (18.1%) produced ESBL. The resistance genes found through PCR were: sul1 - 50%, sul2 86.4%, tetA 42.1%, tetB 68.4%, tetC- 5.3%, blaCTX-M 26.1%, blaTEM -78.3%, blaSHV 4.4%, and intl 35.7%. The knowledge about resistance profile´s and ESBL production is very important in guiding the most appropriate treatment when it is necessary, in addition to knowing the resistance potential that could be transferred to other bacteria.
 
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Release Date
2020-02-23
Publishing Date
2018-02-23
 
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