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Doctoral Thesis
DOI
10.11606/T.87.2012.tde-26112012-094603
Document
Author
Full name
Luiz Gustavo Bentim Góes
Institute/School/College
Knowledge Area
Date of Defense
Published
São Paulo, 2012
Supervisor
Committee
Jerez, José Antonio (President)
Franco, Milene Tino de
Gilio, Alfredo Elias
Oliveira, Maria Isabel de
Passos, Saulo Duarte
Title in Portuguese
Caracterização molecular de coronavírus humano - HCoV, circulantes no município de São Paulo, São Paulo, Brasil.
Keywords in Portuguese
Coronavirus
Crianças
Infecções respiratórias
Viroses
Abstract in Portuguese
As infecções respiratórias agudas (IRA) são as doenças infecciosas mais frequentes em seres humanos e os vírus respiratórios são os agentes de maior ocorrência na etiologia das mesmas. Os coronavírus humanos (HCoVs) são reconhecidos como causa comum de infecções do trato respiratório superior e, menos comumente, do trato respiratório inferior. Dados da ocorrência do HCoV na população brasileira são escassos. O presente estudo tem por objetivo avaliar a ocorrência de HCoV em crianças acometidas por IRA atendidas no Hospital Universitário da Universidade de São Paulo , São Paulo-SP, entre o período de 1995 a 2008. Amostras em cDNA foram utilizadas em ensaio de PCR em Tempo Real para detecção dos 4 tipos de coronavírus. Amostras positivas foram utilizadas em ensaio de PCR convencional e posteriormente tipificadas por sequenciamento. Amostras negativas pelo método de PCR convencional foram tipificadas através de PCR em Tempo Real e Nested PCR específico para cada tipo de HCoV. Quatrocentos e dez amostras foram positivas pelo ensaio de PCR Real Time, sendo o coronavírus detectado em 8.94% das amostras. Duzentos e noventa e oito amostras foram tipificadas, sendo o tipo OC43 o de maior ocorrência, seguido pelos tipos NL63; HKU1 e 229E.
Title in English
Molecular characterization of human coronavirus - HCoV, circulating in São Paulo, São Paulo, Brazil.
Keywords in English
Children
Coronavirus
Respiratory infections
Viruses
Abstract in English
Acute respiratory infections (ARI) are the most common infectious diseases in humans and respiratory viruses are the most frequent agents in the etiology of the same. The human coronaviruses (HCoVs) are recognized as a common cause of upper respiratory tract infections and, less commonly, lower respiratory tract. Data from the occurrence of HCoV in the Brazilian population are scarce, despite the high incidence of respiratory infections during the winter. This study aims to evaluate the occurrence of HCoV in children affected by acute respiratory infections treated at University Hospital of São Paulo (USP), São Paulo-SP, between the periods 1995 to 2008. Samples of cDNA were screened by Real Time PCR for detection of the four types of coronavirus. Positive samples were used in standard PCR assay and subsequently typed by sequencing. Samples positive by Real Time but negative by standard PCR assay were typed by specific Nested PCR and Real-Time for each type of HCoV. Four hundred and ten samples were positive by Real Time PCR assay, and the occurrence of coronaviruses in our samples of 8.94%. Two hundred and ninety eight were typed, the type OC43 being the most frequent, followed by the recently discovered types, NL63 ; HKU1 and HCoV-229E.
 
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Publishing Date
2012-12-18
 
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