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Master's Dissertation
DOI
10.11606/D.87.2011.tde-27092011-104745
Document
Author
Full name
Priscila Comone
Institute/School/College
Knowledge Area
Date of Defense
Published
São Paulo, 2011
Supervisor
Committee
Botosso, Viviane Fongaro (President)
Castrignano, Silvana Beres
Jerez, José Antonio
Title in Portuguese
Diversidade genética da hemaglutinina (HA) de vírus influenza A, entre 1995 e 2006
Keywords in Portuguese
Orthomixoviridae
Evolução molecular
Genoma
Influenza
Sequenciamento genético
Virologia molecular
Abstract in Portuguese
Os Influenzavirus podem ser classificados de acordo com suas glicoproteínas externas hemaglutinina (HA) e neuraminidase (NA), ambas apresentando alta variabilidade genética e antigênica. No presente estudo foi realizada análise molecular do gene HA, do vírus influenza A (IA) em amostras colhidas de crianças e lactentes com sintomatologia respiratória atendidas no Hospital Universitário da Universidade de São Paulo (USP), durante os anos de 1995 a 2006. Um total de 3.009 amostras foram analisadas por duplex RT-PCR e 4,38% (n=132) foram positivas, sendo 12,1% (n=16) Influenza B e 87,9% (n=116) IA, das quais 9% (n=9) eram H1N1, 91% (n=91) eram H3N2 e 13,8% (n=16) não foram subtipadas. A região HA1 do gene HA de 39 amostras foi sequenciada e as sequências comparadas com as cepas vacinais e circulantes dos respectivos anos. A região de ligação ao receptor foi conservada em todas as amostras e foram verificadas alterações de aminoácidos principalmente nos sítios antigênicos e arredores. No geral, as cepas vacinais foram compatíveis com as circulantes em São Paulo.
Title in English
Genetic diversity of hemagglutinin (HA) of Influenza A virus from 1995 to 2006.
Keywords in English
Orthomixoviridae
Genetic sequencing
Genome
Influenza
Molecular evolution
Molecular virology
Abstract in English
The Influenzavirus can be classified according to their external glycoproteins hemagglutinin (HA) and neuraminidase (NA), both showing high genetic and antigenic variability. In the present study was carried out molecular analysis of the HA gene of influenza A (IA) in samples harvested from children and infants, with respiratory symptoms attended at University Hospital, University of Sao Paulo (USP), during the years 1995 to 2006. A total of 3,009 samples were analyzed by duplex RT-PCR and 4.38% (n = 132) were positive, being 12.1% (n = 16) Influenza B and 87.9% (n = 116) IA, where which 9% (n = 9) were H1N1 and 91% (n = 91) were H3N2 and 13.8% (n = 16) did not subtyped. The HA1 region of HA gene of 39 samples were sequenced and the sequences compared with vaccine strains and circulating strains in those years. The receptor-binding region was conserved in all samples and aminoacid changes were observed mainly in the antigenic sites and surroundings. Overall, the vaccine strains were consistent with those circulating in Sao Paulo.
 
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Publishing Date
2011-09-29
 
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