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Master's Dissertation
DOI
10.11606/D.87.2011.tde-30052012-091507
Document
Author
Full name
Diana Maria Chica Cardona
Institute/School/College
Knowledge Area
Date of Defense
Published
São Paulo, 2011
Supervisor
Committee
Schneider, Rene Peter (President)
Gomez, José Gregorio Cabrera
Sato, Maria Inês Zanoli
Title in Portuguese
A procura de bactérias degradadoras de metamidofós.
Keywords in Portuguese
Biodegradação ambiental
Cinética do progresso anaeróbio
Compostos de fósforo
Metamidofos
Microbiologia ambiental
Organofosforados
Abstract in Portuguese
O metamidofós é um inseticida organofosforado altamente tóxico por ser um forte inibidor da acetilcolinesterase, sendo utilizado em diversas culturas para o controle de pragas. Há poucas informações sobre a biodegradação deste composto disponíveis na literatura. Foram isoladas e caracterizadas 12 bactérias a partir de amostras de solo e água de uma área contaminada com metamidofós, as quais mostraram inicialmente capacidade de degradar o pesticida, utilizando-o como fonte de enxofre/nitrogênio e fósforo/enxofre. Estes isolados foram identificados por métodos de biologia molecular e pela caracterização do perfil lipídico da célula como pertencentes aos gêneros, Serratia, Pseudomonas, Stenotrophomonas e Curtobacterium. Ensaios preliminares da cinética de degradação do metamidofós por GC/MS evidenciaram o consumo do pesticida pelas bactérias isoladas. A retomada destes ensaios após alguns meses de armazenamento em glicerol a -80ºC resultou na perda da capacidade de biodegradação do composto-alvo por causas não-identificadas. Fatores que podem ter contribuído para este resultado negativo incluem eventual perda de plasmídeos com partes das vias de biodegradação ou interferentes utilizados na estabilização do metamidofós.
Title in English
Searching for methamidofos degrading bacteria.
Keywords in English
Environmental degradation
Environmental microbiology
Kinetics of progress anaerobic
Methamidophos
Organophosphate
Phosphorus compounds
Abstract in English
Methamidophos is a strong acetylcholinesterase inhibitor and, therefore, a very toxic organophosphorus insecticide. This product has been widely employed for pest control in a variety of cultures, but little information is available about its biodegradation. 12 bacteria were isolated and characterized, from water and soil samples obtained from a site contaminated with methamidophos, which in preliminary tests showed the ability to degrade methamidophos, using it as a combined source of sulfur/nitrogen and/or phosphorus/sulfur. These isolates were identified by molecular biology methods and by characterization of its fatty acids profile as members of the genus Serratia, Pseudomonas, Stenotrophomonas and Curtobacterium. The ability to biodegrade the compound was lost after prolonged storage at -80ºC for unknown reasons. It was hypothesized that this negative result may have occurred due to loss of plasmids or by interference of products used in the stabilization of commercial methamidophos formulations.
 
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Publishing Date
2012-06-27
 
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