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Doctoral Thesis
DOI
10.11606/T.87.2010.tde-31082010-103629
Document
Author
Full name
Valéria Karla de Brito Vieira
Institute/School/College
Knowledge Area
Date of Defense
Published
São Paulo, 2010
Supervisor
Committee
Lima, Lucymara Fassarela Agnez (President)
Ferreira, Rita de Cassia Cafe
Galhardo, Rodrigo da Silva
Schenberg, Ana Clara Guerrini
Silva, Luiziana Ferreira da
Title in Portuguese
Estratégias para obtenção de mutantes de Chromobacterium violaceum e sua caracterização.
Keywords in Portuguese
Chromobacterium violaceum
Biologia molecular
Biotecnologia
Genética bacteriana
Mutação
Violaceína
Abstract in Portuguese
Chromobacterium violaceum tem sido muito estudada a fim de explorar seu potencial biotecnológico, especialmente, a violaceína. Este trabalho teve por objetivo estabelecer estratégias para obtenção de mutantes. Linhagens mutantes foram obtidas por transposição com o mini-Tn5 e por recombinação homóloga (mutantes vioB e ubiB). O vioB em fase estacionária demonstrou ser mais resistente a UVC e aos sais metálicos FeCl2 e CdCl2. Com H2O2 não houve uma diferença significativa entre as taxas de sobrevivência exibidas pela linhagem vioB. Análise por microscopia demonstrou maior agregação das células do mutante vioB e uma maior formação de biofilme. O mutante ubiB apresentou crescimento deficiente na presença de 0.4 mg/mL de fenol.
Title in English
Strategies for obtaining mutant strains of Chromobacterium violaceum and their characterization.
Keywords in English
Chromobacterium violaceum
Bacterial genetics
Biotechnology
Molecular biology
Mutation
Violacein
Abstract in English
Chromobacterium violaceum has been extensively studied for its biotechnological potential, especially what concerns violacein. In this work we have as goal to establish strategies for obtaining mutant strains. Mutant strains were obtained by transposition using mini-Tn5 and employing homologous recombination (mutant vioB and ubiB). vioB was shown to be more resistant in stationary phase to UVC and the metallic salts FeCl2 and CdCl2. With H2O2, there was no significant difference in survival rates exhibited by vioB. Microscopy analyses demonstrated a higher cell aggregation by the vioB mutant and a higher formation of biofilm. ubiB mutant presented growth defects in presence 0.4 mg/mL phenol.
 
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Publishing Date
2010-09-16
 
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