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Master's Dissertation
DOI
10.11606/D.88.2009.tde-24092009-144106
Document
Author
Full name
Valquiria da Cruz Rodrigues Barioto
E-mail
Institute/School/College
Knowledge Area
Date of Defense
Published
São Carlos, 2009
Supervisor
Committee
Mascaro, Lucia Helena (President)
Motheo, Artur de Jesus
Porto, Andre Luiz Meleiro
Title in Portuguese
Influência do contra-íon usado na eletrossíntese do polipirrol em sua resposta como biossensor eletroquímico após a imobilização da polifenol oxidase
Keywords in Portuguese
Biossensores
Catecol
Pirogalol
Polifenol oxidase (PFO)
Polipirrol (PPI)
Abstract in Portuguese
Neste trabalho, foram fabricados biossensores amperométricos a partir do uso da polifenol oxidase (PFO), obtida do abacate como fonte enzimática, imobilizada em filmes de polipirrol (PPI) e que foram eletrossintetizados em meio de três diferentes eletrólitos de suporte (NaCl e NaClO4 e NaDDS). O método de imobilização enzimática foi o da adsorção, sendo a solução de enzima adicionada à solução com o pirrol e o eletrólito durante o processo de eletropolimerização. Os filmes de PPI/PFO foram caracterizados por técnicas eletroquímicas, principalmente por voltametria cíclica. A detecção de compostos fenólicos (catecol e pirogalol) foi realizada pela técnica de cronoamperometria após se variar a concentração do analito. A morfologia dos filmes foi estudada por microscopia de força atômica (AFM), sendo observado que a presença da enzima no filme polimérico assim como o uso de diferentes eletrólitos de suporte levou a diferenças na superfície dos filmes. Além disto, verificou-se que o biossensor construído a partir do uso do NaCl, apresentava uma resposta mais eficiente, ou seja, ele foi capaz de detectar catecol e pirogalol em um menor limite de detecção.
Title in English
Influence of counter-ion used in the electrosynthesis of polypyrrole in your response as electrochemical biosensors after polyphenol oxidase immobilization
Keywords in English
Biosensors
Cathecol
Polyphenol oxidase (PPO)
Polypyrrole (PPY)
Pyrogallol
Abstract in English
In this study amperometric biosensors were manufactured from the use of polyphenol oxidase (PPO) obtained from avocado as a source of enzyme immobilized in polypyrrole (PPY) films that were electrosynthesized with three different support electrolytes (NaCl, NaClO4 and NaDDS). The method of enzyme immobilization was the adsorption. The PPO was added in the solution containing pyrrole and electrolyte during electropolymerization. The PPY/PPO films were characterized by electrochemical techniques mainly by cyclic voltammetry. Detection of phenolic compounds (catechol and pyrogallol) was performed by the technique of chronoamperometry after varying the concentration of the analyte. The morphology of the films was studied by the atomic force microscopy (AFM) and observed that the presence of the enzyme in the polymer film and the use of different electrolytes support led to differences in the surface of films. However it was found that the biosensor constructed from the use of NaCl showed more efficient response and it was able to detect catechol and pyrogallol in a lower limit of detection.
 
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Publishing Date
2010-03-25
 
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