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Doctoral Thesis
DOI
Document
Author
Full name
Silvia Beserra Nogueira
E-mail
Institute/School/College
Knowledge Area
Date of Defense
Published
São Paulo, 2010
Supervisor
Committee
Nascimento, João Roberto Oliveira do (President)
Chillet, Marc
Gozzo, Fábio Cesar
Labate, Carlos Alberto
Purgatto, Eduardo
Title in Portuguese
Análise diferencial do proteoma da polpa do mamão durante o amadurecimento utilizando eletroforese bidimensional
Keywords in Portuguese
Amadurecimento dos frutos
Carica papaya L.
DIGE
Proteinas
Proteoma diferencial
Abstract in Portuguese
O mamão papaia (Carica papaya L.) é uma fruta tropical de grande relevância comercial uma vez que o Brasil é o maior produtor mundial e terceiro maior exportador da fruta. Contudo, por ser uma fruta climatérica, tem uma vida pós-colheita limitada, devido ao rápido Amaciamento da polpa. Neste trabalho foi realizada uma investigação proteômica comparativa de polpa do mamão verde e maduro. Várias centenas de componentes protéicos (spots) foram resolvidos em géis 2-DE (faixa de pH 4-7) utilizando a eletroforese diferencial em gel (DIGE) e posteriormente as imagens geradas foram analisadas pelo programa PDQuest. Os spots diferencialmente expressos foram retirados do gel, digeridos e sequenciados (ESI-Q-TOF-MS/MS). Em geral, as proteínas diferencialmente expressas foram associadas ao metabolismo do etileno, resposta ao estresse, metabolismo de carbono e outros importantes processos fisiológicos. Os papéis de algumas das proteínas identificadas foram discutidos em relação à qualidade dos frutos do mamão. Este estudo fornece a primeira caracterização das mudanças no proteoma da polpa do mamão durante o amadurecimento. Deste modo a identificação de proteínas envolvidas na instalação ou desenvolvimento do amadurecimento pode contribuir para o entendimento deste processo e, também, gerar subsídios para avanços tecnológicos visando à qualidade e diminuição das perdas pós-colheita.
Title in English
Differential analysis of papaya fruit proteome during ripening using two-dimensional electrophoresis
Keywords in English
Carica papaya L
Differential proteome
DIGE
Fruit ripening
Proteins
Abstract in English
Papaya (Carica papaya L.) fruit is a relevant tropical crop since Brazil is the world's largest producer and third largest exporter of fruit. However being a climacteric fruit, has a limited green life due to the rapid pulp softening. We report here a comparative proteomic investigation of fruit pulp from unripe and ripe papayas. Several hundreds of protein components were resolved on 2-DE gels (pH range 4-7) using the differential gel electrophoresis (DIGE) approach, and images were analyzed by PDQuest. The variable components were excised, in-gel digested and were analyzed by ESI-Q-TOF-MS/MS. In general, the differentially expressed proteins were associated to ethylene metabolism, stress response, carbon metabolism and other important physiological processes. The roles of some of the identified proteins were discussed in relation to papaya fruit quality. To the best of our knowledge, this investigation provides the first characterization of the changes in papaya fruit pulp proteome during ripening. Thus the identification of proteins involved in the installation or development of the ripening may contribute to the understanding of this process and also provide subsidies for technological advances aimed at quality and reduction of post harvest losses.
 
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TESE_SBN_FINAL.pdf (7.98 Mbytes)
Publishing Date
2013-07-23
 
WARNING: The material described below relates to works resulting from this thesis or dissertation. The contents of these works are the author's responsibility.
  • NOGUEIRA, Silvia Beserra, et al. Proteomic analysis of papaya fruit ripening using 2DE-DIGE [doi:10.1016/j.jprot.2011.11.015]. Journal of Proteomics [online], 2012, vol. 75, n. 4, p. 1428-1439.
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