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Doctoral Thesis
DOI
10.11606/T.9.2017.tde-22082017-093259
Document
Author
Full name
Maria de Lourdes Reis Giada
E-mail
Institute/School/College
Knowledge Area
Date of Defense
Published
São Paulo, 2006
Supervisor
Committee
Mancini Filho, Jorge (President)
d'Arce, Marisa Aparecida Bismara Regitano
Fett, Roseane
Gonçalves, Lireny Aparecida Guaraldo
Marquez, Ursula Maria Lanfer
Title in Portuguese
Avaliação da capacidade antioxidante dos compostos fenólicos do cotilédone da semente de girassol (Helianthus annuus L.) rajada
Keywords in Portuguese
Ácidos graxos
Antioxidantes
Antioxidantes naturais
Atividade antioxidante
Compostos fenólicos
Métodos analíticos
Nutrição experimental
Abstract in Portuguese
No presente trabalho foi avaliada a capacidade antioxidante in vitro dos extratos do cotilédone da semente de girassol rajada, obtidos por extração seqüencial com solventes de diferentes polaridades, bem como avaliado o potencial antioxidante in vitro do extrato que apresentou maior capacidade in vitro. Todos os parâmetros in vitro (sistema β-caroteno/ácido Iinoléico, métodos FRAP, DPPH, ORAC e Rancimat) indicaram o extrato aquoso (EAq) como o de maior capacidade. Neste extrato, o ácido clorogênico (12,88%) foi identificado como o principal componente dos ácidos fenólicos. Na avaliação da capacidade antioxidante in vitro, ambas as determinações empregadas (TBARS e perfil de ácidos graxos) indicaram o EAq como capaz de exercer um efeito protetor sobre os ácidos graxos poliinsaturados dos tecidos adiposo, cerebral, hepático e plasmático de ratos Wistar machos recém-desmamados.
Title in English
Evaluation of the antioxidant capacity of cotyledon phenolic compounds of sunflower seed (Helianthus annuus L.)
Keywords in English
Analytical Methods
Antioxidant Activity
Antioxidants
Experimental Nutrition
Fatty Acids
Natural Antioxidants
Phenolic Compounds
Abstract in English
The aims of this work were to evaluate the in vitro antioxidant capacity of listed sunflower cotyledon extracts, obtained by a sequential extraction with solvents of different polarities, and to evaluate the in vitro antioxidant potential of the sample extract with highest in vitro capacity. Ali the in vitro parameters (β-carotene/linoleic acid system, FRAP, OPPH, ORAC and Rancimat methods) indicated the aqueous extract (EAq) as the extract with highest capacity. In this extract, the chlorogenic acid (12.88%) was identified as the principal fraction of phenolic acids. In the in vitro antioxidant capacity evaluation, both determinations used (TBARS and fatty acids profile) gave indication that the EAq was capable to exerce a protective effect on the polyunsaturated fatty acids of the adipose, cerebral, hepatic and plasm tissues of Wistar male rats just-weaned.
 
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Publishing Date
2017-08-22
 
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