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Master's Dissertation
DOI
10.11606/D.9.2004.tde-28012005-111217
Document
Author
Full name
Carin Gorescu Caldeira
E-mail
Institute/School/College
Knowledge Area
Date of Defense
Published
São Paulo, 2004
Supervisor
Committee
Melles, Carmo Elias Andrade (President)
Vaz, Adelaide José
Zanella, Rosemeire Cobo
Title in Portuguese
Resposta imune humoral de pacientes com doença meningocócica frente a lipooligossacarídeos específicos
Keywords in Portuguese
Formação de anticorpos
Lipopolissacarídeo
Neisseria meningitidis
Abstract in Portuguese
A meningite meningocócica permanece como importante causa de morbidade e mortalidade em todo o mundo. Extratos purificados de LOS de Neisseria meningitidis foram utilizados em um ensaio de ELISA a fim de detectar a especificidade dos anticorpos anti-LOS produzidos por 41 pacientes com doença meningocócica. Durante o período de convalescença, 100% dos pacientes apresentaram aumento da concentração sérica de IgG anti-LOS (L1, L2, L3,7, L6, L8 e L10) em no mínimo três vezes a concentração verificada nos soros coletados durante a fase aguda. O maior aumento foi de oito vezes para IgG anti-L3,7 adicionada de resíduo de ácido siálico. Portadores de Neisseria meningitidis e Neisseria lactâmica na nasofaringe não produziram anticorpos anti-LOS. Através de um ensaio ELISA de Inibição, foi possível verificar a especificidade dos anticorpos anti-LOS, exceto para os anticorpos IgG anti-L10, que apresentaram reação cruzada com outros tipos antigênicos de LOS como L2, L3,7 e L6, revelando-se um importante candidato a antígeno vacinal.
Title in English
Humoral immune response of patients with meningococcal disease to specific lipopolisaccharides
Keywords in English
Antibody
Lipopolisaccharide
Neisseria meningitidis
Abstract in English
Meningococcal meningitis remains as an important cause of morbidity and mortality all over the world. Purified LOS extracts were used in an ELISA assay to verify the specificity of the antibodies produced due to meningococcal disease. All 41 convalescent sera collected from patients had at lowest a three-folder increase of anti-LOS IgG concentrations when compared with acute sera. A higher increase of eight fold was seen to anti-L3,7 IgG specific antibodies. Safe carries of Neisseria meningitidis and Neisseria lactamica had no detectable specific anti-LOS antibodies. In an Inhibition ELISA assay, except for anti-L10 IgG, antibodies against L1, L2, L3,7, L6 and L8 immunotypes were specific. Anti-L10 antibodies cross-reacted with L2, L3,7 and L6 epitopes.
 
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Publishing Date
2013-04-03
 
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