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Master's Dissertation
DOI
10.11606/D.9.2005.tde-21082007-160740
Document
Author
Full name
Carolina Dizioli Rodrigues de Oliveira
E-mail
Institute/School/College
Knowledge Area
Date of Defense
Published
São Paulo, 2005
Supervisor
Committee
Moreau, Regina Lúcia de Moraes (President)
Malbergier, André
Silva, Ovandir Alves
Title in Portuguese
Determinação de canabinóides em cabelo por microextração em fase sólida por Headspace e análise por espectrometria de massa associada à cromatografia em fase gasosa
Keywords in Portuguese
Cabelo
Canabinóides
Cannabis
Cromatografia em fase gasosa associada a espectrometria de massa
Microextração em fase sólida por headspace
Abstract in Portuguese
Foi desenvolvido um método para determinar canabinóides (canabidiol, canabinol e delta-9-tetraidrocanabinol) no cabelo. Uma amostra de 10mg foi descontaminada com diclorometano, seguida de digestão alcalina, microextração em fase sólida por headspace (HS-SPME) e analisada por espectrometria de massa associada à cromatografia em fase gasosa (GC/MS). Os limites de detecção e de quantificação foram de 0,07 e 0,12 ng/mg, respectivamente, para todos canabinóides estudados. O método demonstrou ser simples, rápido, preciso e linear no intervalo de 0,12 a 12 ng/mg (r2 > 0,98). Amostras de cabelo de 8 usuários de Cannabis foram coletadas de pacientes provenientes de uma clínica dependentes pela equipe médica. O método mostrou-se eficiente em amostras de cabelos de usuários que faziam uso da droga pelo menos 10 vezes por semana.
Title in English
Determination of cannabinoids in hair by Headspace solid-phase microextraction and gas chromatography-mass spectrometry
Keywords in English
Canabinnoids
Cannabis
Gas chromatography-mass spectrometry
Hair
Headspace solid-phase microextraction
Abstract in English
A method was to develop to detect cannabinoids (cannabidiol, cannabinol and delta-9-tetrahydrocannabinol) in hair. A 10 mg of hair sample was descontaminated by dichloromethane followed by alkalin digestion, headspace solid-phase microextraction technique (HS-SPME) and analyzed by gas chromatography-mass spectrometry (CG/MS). The detection and quantitation limits were 0,07 and 0,12ng/mg respectively for all studied cannabinoids. The method proved to be simple, fast, precise and linear at the range of 0,12 to 12ng/mg (r2 > 0,98). Eight hair samples of Cannabis user were collected from patients at admittance from a dependence clinic by clinical staff. The method showed efficient in samples of users who use the drug at least 10 fold a week.
 
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Publishing Date
2007-09-28
 
WARNING: The material described below relates to works resulting from this thesis or dissertation. The contents of these works are the author's responsibility.
  • DE OLIVEIRA, Carolina Dizioli Rodrigues, YONAMINE, Mauricio, and MOREAU, Regina Lucia de Moraes. Headspace solid-phase microextraction of cannabinoids in human head hair samples [doi:10.1002/jssc.200600192]. Journal of Separation Science [online], 2007, vol. 30, n. 1, p. 128-134.
All rights of the thesis/dissertation are from the authors
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