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Doctoral Thesis
DOI
Document
Author
Full name
Daiana Rodrigues Torres
E-mail
Institute/School/College
Knowledge Area
Date of Defense
Published
Lorena, 2019
Supervisor
Committee
Roberto, Inês Conceição (President)
Antonini, Sandra Regina Ceccato
Silva, João Paulo Alves
Silva, Roberto da
Title in Portuguese
Aproveitamento do Hidrolisado da casca de mandioca como substrato para a produção de carotenoides por leveduras isoladas da Região Amazônica
Keywords in Portuguese
anti-oxidante
Casca de mandioca
Leveduras
Pigmentos carotenoides
Vitamina A
Abstract in Portuguese
O presente trabalho teve como objetivo o aproveitamento do hidrolisado de cascas de mandioca para produção de pigmentos carotenoides. Para isso, foi realizado o isolamento de leveduras provenientes da Região Amazônica, caracterização das cascas de mandioca e obtenção dos hidrolisados por via ácida (HA) e enzimática (HE). Por fim, foram avaliados o efeito da relação C:N e da luminosidade sobre a produção de carotenoides pelas leveduras previamente selecionadas nos hidrolisados. Os extratos carotenogênicos foram caracterizados quanto ao potencial anti-oxidante e antimicrobiano, sendo ainda avaliado o uso da biomassa seca como agente pigmentante em um material polimérico. Foram isoladas sete colônias pigmentadas (4 do solo, 2 de água e 1 de fruto), as quais foram identificadas como pertencentes ao gênero Rhodotorula. Com relação as cascas de mandioca, o principal componente em % m/m foi o amido (71,0), seguido da lignina (13,0), glucana (4,6), xilana (2,4), cinzas (2,6) e extrativos (4,6). Na melhor condição de hidrólise ácida (1% H2SO4, 10% sólidos, 120 min), foi obtido 48 g/L de glicose, o que correspondeu a uma eficiência de 67%. Já para a hidrólise enzimática (3000 U Termamyl 2X, 240 U AMG XXL, 14% sólidos, 65 min) obtevese 80,8 g/L de glicose, correspondendo a uma eficiência de 79,5%. Nas condições de cultivo empregando HA ou HE contendo cerca de 50,0 g/L de glicose sem qualquer suplementação (pH inicial 6,0, temperatura de 30ºC, agitação de 200rpm e na presença de luminosidade) foi possível selecionar a cepa Rh S2 no HA e a Rh RNA no HE, as quais apresentaram, respectivamente (12,7 g/L e 7,6 mg/L) e (22,8 g/L e 11,2 mg/L) de biomassa e carotenoides totais. O maior potencial antioxidante foi obtido com a cepa Rh S2 que apresentou cerca de 30% de redução do radical DPPH sendo que ambos extratos apresentaram ação anti-microbiana frente à Escherichia coli e Aspergillus fumigatus. Quanto as propriedades mecânicas do compósito formulado com a biomassa seca da cepa Rh S2, ficou demonstrada a possibilidade do uso desta biomassa como agente pigmentante em resinas poliméricas. Estes resultados revelam o grande potencial das leveduras selecionadas para a produção de carotenoides a partir de um subproduto da agroindústria, ressaltando ainda suas importantes propriedades biológicas e de pigmentação, as quais poderão ser exploradas em diferentes aplicações.
Title in English
Utilization of the cassava peel hydrolyzate as a substrate for the production of carotenoids by yeasts isolated from the Amazon Region
Keywords in English
antioxidant
Carotenoid pigments
Cassava peel
Vitamin A
Yeasts
Abstract in English
The present work had as objective the utilization of the hydrolyzate of cassava peels for the production of carotenoid pigments. For this, the yeast from the Amazon region was isolated, characterizing the cassava peels and acid hydrolysates (HA) and enzymatic (HE). Finally, the effect of the C: N ratio and the luminosity on carotenoid production by the selected yeasts in the hydrolysates were evaluated. Carotenogens extracts are characterized as antioxidant and antimicrobial potential, and the use of dry biomass as a pigment agent in a polymeric material is also evaluated. Seven pigmented colonies (4 soil, 2 water and 1 fruit) were isolated, as were the communities as individuals belonging to the genus Rhodotorula. Compared with cassava peels, the main component in% by weight of starch (71.0), followed by lignin (13.0), glucan (4.6), xylan (2.4), ashes (26) and extractives (4.6). The acid hydrolysis condition (1% H2 SO4, 10% solids, 120 min) was 48 g/L glucose, corresponding to an efficiency of 67%. Already for an enzymatic hydration (3000 U Termamyl 2X, 240 U AMG XXL, 14% solids, 65 min), 80.8 g/L of glucose was obtained, corresponding to an efficiency of 79.5%. In the ingestion conditions using HA or HE of about 50.0 g/L of glucose without any supplement (initial pH 6.0, temperature of 30 ° C, agitation of the presence light) it was possible to select the strain Rh S2 in HA and Rh RNA is not HE, as it is today (12.7 g/L and 7.6 mg/L) and (22.8 g/L and 11.2 mg/L) biomass and total carotenoids. The highest antioxidante potential was obtained with the Rh S2 protein which had about 30% reduction of the DPPH radical, with both extracts being moved against the microbiota against Escherichia coli and Aspergillus fumigatus. Regarding the mechanical properties of the compound formulated with a dry biomass of the strain Rh S2, the possibility of the use of this biomass as pigment agent in polymer resins has been demonstrated. This research was useful to increase the potential of selected yeasts for the production of carotenoids from a by-product of agroindustry, highlighting their historical and pigmentation characteristics, such as the ports explored in different applications.
 
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Release Date
2021-06-16
Publishing Date
2019-06-17
 
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